胃癌幽门螺杆菌感染中c-met基因表达与增殖的关系及其预后

目的 研究c-met基因蛋白及增殖细胞核抗原（PCNA）在幽门螺杆菌（Hp)感染的胃黏膜病变演进中的表达及关系，探讨Hp感染对胃癌预后的意义。方法 采用免疫组织化学法检测145例经病理证实不同胃黏膜病变的c-met和PCNA基因表达，Warthin-Starry法检测Hp感染。结果 在浅表性胃炎（CSG）、萎缩肠化性胃炎、异型增生（DYS）、早期胃癌和进展期胃癌中，c-met和PCNA2种基因在萎缩肠化性胃炎、DYS、胃癌均显著高于CSG（P＜0．05）。对胃黏膜增殖程度与c-met和PCNA阳性表达强度的密切关系分析，表明两者有显著关联（P＜0．01）。c-met和PCNA阳性表达与胃癌组织类型、浆膜浸润和淋巴结转移密切相关，而且Borrmann Ⅳ明显高于早期胃癌（P＜0．05）。c-met－LI和PCNA－LI在胃癌中等级相关表达有极显著的相关性（P＜0．001）。c-met阳性表达与肠型胃癌Hp感染有关。萎缩肠化性胃炎、DYS和胃癌组c-met阳性表达中Hp感染者明显高于阴性者。Hp阳性者5年生存期显著短于Hp阴性者。结论 c-met和PCNA基因表达与胃黏膜增殖和恶化有关，c-met基因可能成为评估胃癌恶化和预后的1项新的重要指标。Hp感染和c-met表达与胃黏膜增殖和恶化有关，Hp感染与胃癌预后有关。

The prognostic research of expression and relationship between Helicobacter pylori of c-met oncogene correlation with gastric mucosal proliferation

Objective To determine whether there is a relationship between Helicobacter pylori (Hp)and expression of c met oncogene and proliferating cell nuclear antigen (PCNA) in gastric mucosal lesions, and prognostic significance of gastric carcinomas(GC). Methods Expression of c met and PCNA were investigated in 145 gastric mucosal lesions by using immunohistochemistry. Hp was examined by Warthin Starry method.Results For chronic superficial gastritis(CSG), chronic atrophic gastritis and intestinal mataplasia (CAS IM), dysplasia(DYS), early GC and advanced GC, the positive rates of c met and PCNA were higher in IM, DYS and GC than in CSG ( P <0.05). Expressions of c met and PCNA increased with the increasing proliferation of gastric mucosa,in rank correlation test,they had a close relationship ( P <0.01).Expressions of c met and PCNA were correlated significantly with histologic type, serosal invasion and lymph node metastasis. In particular, the expression of c met and PCNA were significantly higher in Borrmann type 4 GC, type 3 GC and type 1,2 than in early GC( P <0 05). By rank correlation test, c met and PCNA have a close relationship ( P <0.001).Expressions of c met of Hp positive were higher than Hp negative in intestinal GC ( P <0.05). Expressions of c met were correlated significantly with Hp positive in CAG IM, DYS and GC. The survival rate of patients with Hp infection was significantly poorer than that of patients with Hp non infection.Conclusions The expression of c met may be is associated with proliferation and malignant transformation of gastric mucosa. These results suggest that expression of c met may be a new prognostic factor in gastric carcinoma. Hp infection and expression of c met may be associated with in proliferation and malignant transformation of gastric mucosa. Hp infection is associated with prognosis of gastric carcinoma.