环氧化物水解酶基因多态性与慢性苯中毒易感性关系的研究

目的　探讨环氧化物水解酶 (mEH)的基因多态性和慢性苯中毒易感性的关系。方法　采用病例 -对照研究 ,以 15 2名苯中毒工人为病例组 ,15 2名接触苯而没有中毒表现的工人为对照组。应用多聚酶链反应 -限制性长度多态性分析技术 (PCR RFLP)检测mEH基因c .113和c.139位点的多态性。结果　携带mEHc .113C C基因型的个体发生苯中毒的危险性为携带有mEHc .113T T和T C基因型个体的 0 .6 0 (OR =0 .6 0 ,95 %CI:0 .37～ 0 .97,P =0 .0 4 ) ;在不吸烟的人群中 ,携带mEHc .113C C基因型的个体苯中毒的发病风险为携带T T和T C基因型的个体 0 .5 6 (OR =0 .5 6 ,95 %CI:0 .33～ 0 .96 ,P =0 .0 3) ;在不饮酒的人群中 ,携带有mEHc .113C C基因型又不饮酒的个体发生苯中毒的危险性为携带有mEHc .113T T和T C基因型的个体 0 .5 1(OR =0 .5 1,95 %CI:0 .30～ 0 .86 ,P =0 .0 1)。结论　携带有mEHc .113C C基因型 ,同时又不吸烟或不饮酒的个体发生慢性苯中毒危险性较低。

Relationship of genetic polymorphism of microsomal epoxide hydrolase with susceptibility of chronic benzene poisoning

OBJECTIVE: To explore the relationship between genetic polymorphisms of microsomal epoxide hydrolase (mEH) and susceptibility of chronic benzene poisoning (BP). METHOD: A case-control study was conducted. 152 BP patients and 152 workers occupationally exposed to benzene without poisoning manifestations were investigated. Polymerase chain reaction-restrained fragment length polymorphism technique (PCR-RFLP) was applied to detect the single nucleotide polymorphisms (SNPs) on c.113 and c.139 of mEH gene. RESULTS: The risk of BP for individuals carrying mEHc.113 C/C genotype was 0.60 (OR = 0.60, 95% CI: 0.37 - 0.97, P = 0.04) of those carrying T/T and T/C genotypes. In non-smoking population, the risk of BP for subjects carrying mEHc.113 C/C genotype was 0.56 (OR = 0.56, 95% CI: 0.33 - 0.96, P = 0.03) of those carrying T/T and T/C genotypes, and in non-drinking population, the individuals carrying mEHc.113 C/C genotype was 0.51 (OR = 0.51, 95% CI: 0.30 - 0.86, P = 0.01) of those carrying T/T and T/C genotypes. CONCLUSION: The subjects carrying mEHc.113 C/C genotype and together with non-smoking or non-drinking habit may have lower risk of chronic benaene poisoning.