睡眠剥夺对大鼠不同脑区 syp 表达的影响

目的：观察睡眠剥夺对大鼠不同脑区神经元细胞syp表达的影响。方法20只SD大鼠随机分为对照组（10只）和模型组（10只）。模型组大鼠利用水上站立法建立睡眠剥夺大鼠模型，分别在睡眠剥夺24和72 h时随机取5只大鼠，处死，通过免疫组化方法观察大鼠不同脑区神经元细胞syp表达变化，对照组不做任何处理，其余同模型组。结果模型组大鼠海马区、前额叶皮质神经元syp表达明显低于对照组，差异显著，而两组纹状体区神经元syp表达差异不显著。结论睡眠剥夺后可导致大鼠皮层和海马神经元syp表达明显降低，对纹状体区神经元syp表达无影响。

Effect of sleep deprivation on the expression of syp in different brain area of rats

Objective To investigate the effects of sleep deprivation on the expression of SYP in different brain area of rats . Methods 20 sprague dawley rats were randomly divided into control group （n=10） ,model group （n=10） .Sleep deprivation models were established by the modified multiple platform methods ,5 control group rats and 5 sleep deprivation model group rats were randomly selected and sacrificed at 24 hours .The other rats were sacrificed at 72 hours .The expression levels of syp in different region of brain were detected by immunohistochemical .The control group rats were left intact .Results The expres‐sion of syp in hippocampus and prefrontal cortex in model group were much higher than that of control group ,but there was no difference in the expression of syp in corpus striatum of the two groups .Conclusion Sleep deprivation could decrease the ex‐pression of syp in hippocampus and prefrontal cortex ,but had no effect on the expression of syp in corpus striatum .