硝酸盐还原试验与反向斑点杂交试验联合检测结核分枝杆菌耐药性

目的 联合检测结核分枝杆菌耐药性,以提高其灵敏度和特异性.方法 对126株已知耐药浓度的结核分枝杆菌耐药株和敏感株分别采用硝酸盐还原试验( Nitrate Reductase Assay,NRA)和反向斑点杂交试验(reverse-dot blot hybridization,RBH),检测其异烟肼(INH)和对利福平(RFP)的耐药性,并将结果将与绝对浓度法结果相比较.结果 硝酸盐还原试验和反向斑点杂交试验联合检测与绝对浓度法比较有高度的一致性(P＞0.05),异烟肼灵敏度95.5％,特异性100％；利福平灵敏度100％,特异性98.1％.结论 硝酸盐还原试验和反向斑点杂交试验联合检测可作为快速结核分枝杆菌耐药性试验方法.

Joint detection of Mycobacterium tuberculosis drug resistance with Nitrate Reductase Assay and Reverse-dot Blot Hybridization

Objective To establish a joint detection method of Mycobacterium tuberculosis drug resistance to improve the sensitivity and specificity of resistance detection methods.Methods 126 resistant and sensitive strains of MTB were analyzed by Nitrate Reductase Assay(NRA) and Reverse-dot Blot Hybridization(RBH) for isoniazid(INH) and rifampicin(RFP),respectively.The results were compared with absolute drug susceptibility tests.Results The joint detection was relatively high degree of consistency(P>0.05),compared with the nitrate reductase assay,reverse-dot blot hybridization and the joint detection with absolute concentration method.The sensitivity of isoniazid(INH) and rifampicin(RPF) was 95.5%,100%,and the specificity was 100%,98.1%.Conclusion The nitrate Reductase assay and reverse-dot blot hybridization can be used as fast joint detection of Mycobacterium tuberculosis drug resistance.