结核分枝杆菌黏附素(HBHA)对小鼠结核病免疫治疗作用的实验研究

目的 探讨结核分枝杆菌粘附素(HBHA)对小鼠结核病实验模型的免疫治疗作用.方法 取在改良罗氏培养基上生长良好的结核分枝杆菌H37Rv两周培养物,制备成均匀菌悬液,每只小鼠尾静脉注射菌量为106 CFU.于感染2周后,分别对CPG组30 ug、HBHA组5 ug、CpG+ HBHA组(CpG 30 ug和HBHA 5 ug)及对照组腹腔注射生理盐水.于感染后4周脱颈处死小鼠,称取小鼠体重、肺脏和脾脏重量,对肺和脾组织进行菌落计数,观察肺病理改变.结果 各实验组肺组织的重量与体重和对照组相比稍有偏大,菌落计数中发现HBHA免疫治疗组明显优于空白对照组,在病理切片中对照组小鼠肺组织有大片肉芽肿形成,病理损害严重.其它各组肺部病变呈弥散分布,但病变程度明显轻于对照组,在抗酸染色中空白对照组有大量的抗酸杆菌存在,其它各组相对明显减少.结论 实验结果表明HBHA对治疗小鼠结核病变模型起一定的作用.

The immunotherapy of Heparin - binding Haemagglutinin (HBHA) from Mycobacterium tuberculosis on Tuberculosis Mice

Objective To explore whether HBHA can play an immunotherapy role in tuberculosis mice.Methods H37Rv was cultured for 2 weeks and its suspending liquid was counted.The injected bacteria number for per mouse was 106 CFU.After challenging H37Rv 14 days through tail vein,we divided mice into 4 groups including 30 ug CPG,5 ug HBHA,30 ug CPG+5 ug HBHA and saline control groups.Each group had 10 mice and injected H37Rv through abdominal cavity.The mice were killed,and then weighed total mouse and its speen and lung,counted bacteria number of spleen and lung,and observed its histopathology from spleen and lung of mouse after 4 weeks challenged H37Rv.Results Total weight from mouse and its spleen and lung from experimental groups was heavier than that from control group.The bacteria load of spleen and lung from experimental groups was obviously less than that from control group.The result showed from histopathology of spleen and lung of mouse that control group had more severe pathological damage.Conclusions HBHA can play a role in immunological treatment in tuberculosis mice.