茶多酚EGCG对人骨髓基质干细胞成骨分化基因表达的影响

目的:观察茶多酚EGCG对人骨髓基质干细胞成骨分化基因ALK、COL1A1、RUNX2、BMP-2表达的影响.方法:培养人骨髓基质干细胞,用浓度为10-5M、10-6M茶多酚EGCG以及空白对照组分别作用人骨髓基质干细胞21 d后,采用荧光定量PCR的方法检测成骨相关基因ALK、COL1A1、RUNX2、BMP-2的表达变化.结果:BMP-2基因的表达水平为:10-5M茶多酚组>10-6M茶多酚组>DMSO对照组;ALK、RUNX2、COL1A1基因在茶多酚EGCG组与空白对照组之间表达无明显变化.结论:茶多酚EGCG可以提高人骨髓基质干细胞BMP-2基因的表达水平,有可能是茶多酚促进人骨髓基质干细胞成骨分化的重要基因.

Effects of tea polyphenols EGCG on the mRNA expression in the osteogenic differentiation of human bone marrow stromal cells

Objective: To study the effects of tea polyphenols EGCG on osteogenic differentiation of human bone marrow stromal cells by testing the mRNA expression level.Methods: HBMSCs were cultured in vitro.HBMSCs were treated with 10-5mol/L、10-6mol/L tea polyphenols and blank group for 21 days.The relative expression of osteogenic gene ALK、COL1A1、RUNX2、BMP-2 were detected by real-time PCR with SYBR Green I.Results: The BMP-2 mRNA expression level:10-5M tea polyphenols group>10-6M tea polyphenols group>blank group.There was no significant difference in the expression of osteogenic gene ALK、COL1A1、RUNX2.Conclusion:Tea polyphenols EGCG could increase the BMP-2 mRNA expression.It could be the important gene that tea polyphenols enhances osteogenesis in the hBMSCs.