| 酸性染料比色法测定乌头属不同药材中乌头生物碱含量 |
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| 引用本文： | 张敏敏,蔡晓丹,李耿,肖日平,阮永队,马春玲,陈长青,赖小平.酸性染料比色法测定乌头属不同药材中乌头生物碱含量[J].中医药导报,2012,18(8):78-79,82. |
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| 作者姓名： | 张敏敏 蔡晓丹 李耿 肖日平 阮永队 马春玲 陈长青 赖小平 |
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| 作者单位： | 1. 广州中医药大学,广东,广州,510006
2. 塘厦医院,广东,东莞,518110 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)课题，广东省科技计划项目 |
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| 摘 要： | 目的:测定毒性中药附子、川乌、草乌、雪上一枝蒿的乌头生物碱含量,对这4种药材的临床用量进行控制。方法:用酸性染料比色法,测定制附子、川乌、草乌中雪上一枝蒿的乌头生物碱含量。结果:附子中生物碱含量为0.2403 mg/g,川乌为1.0412 mg/g,草乌为2.1149 mg/g,雪上一枝蒿为0.6702 mg/g。结论:本实验中建立的酸性染料比色法测定乌头生物碱含量,可作为评价毒性药材质量的根据。
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| 关 键 词： | 酸性染料比色法 乌头属 生物碱 附子 川乌 草乌 雪上一枝蒿 |
Content Determination of Aconitine-type Alkaloids in Aconitum Different Medicinal Materials by Acid-dye Colormetry |
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| Zhang Minmin
,
Cai Xiaodan
,
Li Geng
,
Xiao Riping
,
Ruan Yongdui
,
Ma Chunlin
,
Chen Changqing
,
Lai Xiaoping.Content Determination of Aconitine-type Alkaloids in Aconitum Different Medicinal Materials by Acid-dye Colormetry[J].GUIDING JOURNAL OF TCM,2012,18(8):78-79,82. |
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| Authors: | Zhang Minmin Cai Xiaodan Li Geng Xiao Riping Ruan Yongdui Ma Chunlin Chen Changqing Lai Xiaoping |
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| Institution: | 1(corresponding author)(1.Guangzhou University of Traditional Chinese Medicine,Guangzhou 510006,China;2.The Hospital of Tangxia,Dongguan 518110,China) |
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| Abstract: | Objective : To determine the content of aconitine-type alkaloids in prepared aconite root,radix aconite,radix aconite kusnezoffii and Radix Aconiti Brachypodi,and to control Clinical dosage.Method: Acid-dye colormetry was employed to determine the content of Aconitine-type Alkaloids in prepared aconite root,radix aconite,radix aconite kusnezoffii and Radix Aconiti Brachypodi.Results : Content of alkaloids in prepared aconite root is 0.2403 mg/g,1.0412 mg/g in radix aconite,2.1149 mg/g in radix aconite kusnezoffii and 0.6702 mg/g in Radix Aconiti Brachypodi.Conclusion : The method to determine content of Aconitine-type Alkaloids in Aconitum different medicinal materials by Acid-dye colormetry can be regarded as standard to evaluate quality of medicinal materials. |
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| Keywords: | Acid-dye colormetry Aconitum alkaloids Aconite root Radix aconite Radix aconite kusnezoffii Radix Aconiti Brachypodi |
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