动脉灌注化疗对术前大肠癌微血管密度的影响

目的 分析术前动脉灌注化疗对大肠癌生物学行为的影响,并探讨术前动脉灌注化疗对肿瘤细胞影响的机制.方法 35例大肠癌患者随机分为术前动脉灌注化疗组(20例)和对照组(15例).术前动脉灌注化疗采用动脉造影技术,经肿瘤营养动脉给予氟尿嘧啶1 000 mg,表阿霉素60 mg,丝裂霉素10 mg.全部患者均接受手术治疗,切除标本分别行常规病理检查及免疫组织化学染色(因子相关抗原染色)测定大肠癌组织微血管密度(MVD).微血管密度计数采用Weidner方法分别计数癌中心、癌黏膜表面及癌旁组织的微血管数.结果 术前动脉灌注化疗组大肠癌组织中心、癌黏膜表面及癌旁组织的MVD分别为(40.46±7.06)、(52.27±18.40)和(49.92±8.15);对照组大肠癌组织中心、癌黏膜表面及癌旁组织的MVD分别为(46.09±12.21)、(73.4±22.06)和(51.94±12.64).两组之间癌黏膜表面的MVD值差异有统计学意义(P＜0.05),癌旁组织及癌中心组织MVD差异无统计学意义.结论 术前动脉灌注化疗可以降低大肠癌组织黏膜表面的微血管密度.

Effects of pre-operative intraarterial infusion chemotherapy on micro-vessel density in colorectal cancer

Objective To evaluate the effects of pre-operative intra-arterial infusion chemotherapy on colorectal Cancer.Methods 35 patients with colorectal cancer,treated by surgery were divided into two groups randomly.There were 20 patients in group A(pre-operative intra-arterial infusion chemotherapy) and 15 in group B(control).Arterial contrast technique Was used in group A then mitromye in 10mg,5-Fu 1000mg epirubic in 60mg were given through Weidner way-Results Microveasel density in the center,surface of the tumor and adjacent tissue around the tunlor were (40.46±7.06),(52.27±18.40),(49.92±8.15) in group A,and (46.09±12.21),(73.44±22.06),(51.94±12.64)in group B.Microvessel density on the surface in group A was significantly lower than that of group B(P＜0.05),with no significance between the center and the adjacent tissue.Conclusion Pre-operative intraarterial infusion chemotherapy is able to reduce micro-vessel density on the surface of eolorectal tumor.