| 重症急性胰腺炎大鼠紧密连接蛋白-1的表达与肠黏膜屏障损伤 |
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| 引用本文： | 曹洪庆,黄鹤光,陈燕昌,李洪森. 重症急性胰腺炎大鼠紧密连接蛋白-1的表达与肠黏膜屏障损伤[J]. 中国普外基础与临床杂志, 2011, 18(3) |
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| 作者姓名： | 曹洪庆 黄鹤光 陈燕昌 李洪森 |
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| 作者单位： | 福建医科大学附属协和医院普外科,福建,福州,350001 |
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| 基金项目： | 福建省自然科学基金(项目编号:2008J0086); 黎介寿院士肠道屏障研究专项基金~~ |
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| 摘 要： | 目的研究紧密连接蛋白-1(ZO-1)在重症急性胰腺炎(SAP)大鼠回肠组织中的表达情况,初步探讨SAP时肠黏膜屏障损伤的可能机理。方法 SD雄性大鼠50只,按完全随机法分为假手术组和SAP组,SAP组按取材时间不同又分为3、6、12及24 h 4个亚组,每组10只。SAP组采用Aho法逆行穿刺胆胰管注射5%牛磺脱氧胆酸钠建立SAP模型,分别于造模后第3、6、12及24 h处死大鼠,假手术组直接处死。建模成功后检测肿瘤坏死因子-α(TNF-α)水平及二胺氧化酶(DAO)活性,同时观察胰腺和回肠组织的病理改变,分别用免疫组织化学蛋白定位及逆转录-聚合酶链反应(RT-PCR)检测回肠组织中ZO-1蛋白和mRNA表达水平。结果与假手术组〔TNF-:α(10.83±0.96)ng/L;DAO:(354.79±3.67)U/L;ZO-1蛋白:(10.40±0.45)分;ZO-1 mRNA:0.878±0.014 8〕相比较,SAP组制模后不同时相大鼠血清中TNF-α水平〔3 h:(125.30±0.94)ng/L;6 h:(181.89±4.93)ng/L;12 h:(230.58±1.28)ng/L;24 h:(198...
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| 关 键 词： | 重症急性胰腺炎 大鼠 紧密连接蛋白-1 肠黏膜屏障 |
Expression of Zonula Occludens-1 and Mucosal Barrier Injury in Rats with Severe Acute Pancreatitis |
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| CAO Hong-qing,HUANG He-guang,CHEN Yan-chang,LI Hong-sen. Expression of Zonula Occludens-1 and Mucosal Barrier Injury in Rats with Severe Acute Pancreatitis[J]. Chinese Journal of Bases and Clinics In General Surgery, 2011, 18(3) |
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| Authors: | CAO Hong-qing HUANG He-guang CHEN Yan-chang LI Hong-sen |
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| Affiliation: | CAO Hong-qing,HUANG He-guang,CHEN Yan-chang,LI Hong-sen.Department of General Surgery,Affiliated Union Hospital of Fujian Medical University,Fuzhou 350001,Fujian Province,China |
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| Abstract: | Objective To study the expression of zonula occludens-1(ZO-1) in ileum tissues and the possible mechanism of intestinal mucosal barrier injury in rats with severe acute pancreatitis(SAP).Methods Fifty SD male rats were randomly divided into sham operation group and SAP group,then SAP group was divided into four subgroups with 10 rats in each subgroup according to the sampling time of 3,6,12,and 24 h.The SAP model was made by injecting 5% bovine sodium deoxycholate into biliary-pancreatic duct with Aho's met... |
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| Keywords: | Severe acute pancreatitis Rat Zonula occludens-1 Intestinal mucosal barrier |
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