MTA1基因调控人前列腺癌PC-3细胞失巢凋亡的研究

目的:探讨MTA1基因小干扰RNA(siRNA)对前列腺癌细胞PC-3增殖和失巢凋亡的影响。方法:应用MTA1 siRNA转染处理人前列腺癌细胞系PC-3后,采用实时定量PCR和W estern印迹检测MTA1基因mRNA和蛋白水平,采用软琼脂集落培养试验检测锚着不依赖性增殖,采用琼脂糖凝胶电泳和流式细胞术检测癌细胞失巢凋亡。结果:与对照组比较,MTA1基因siRNA转染组MTA1 mRNA和蛋白水平明显下降,且呈浓度依赖性(r=0.935,P=0.0001)。MTA1 siRNA转染组软琼脂集落形成数明显减少,且与浓度相关(r=0.901,P=0.0005)。琼脂糖凝胶电泳和流式细胞术结果显示,MTA1 siRNA转染可诱导前列腺癌细胞失巢凋亡,且与浓度相关(r=0.916,P=0.0003)。结论:MTA1 siRNA可抑制人前列腺癌细胞增殖,诱导失巢凋亡是其机制之一。

Regulatory effect of MTA1 on the anoikis of human prostate cancer cells

Objective： To study the effects of MTA1 small interfering RNA（siRNA） on the anchorage-independent growth and anoikis of prostate cancer cell line PC-3.Methods： After transfection of human prostate cancer PC-3 cells by MTA1 siRNA,we detected the expression of the MTA1 gene by real-time PCR and Western blot,the anchorage-independent growth of the cells by clone formation in soft agar,and their anoikis by DNA fragmentation assay and flow cytometry.Results： Compared with the control group,MTA1 siRNA transfection significantly decreased the mRNA and protein levels of MTA1,inhibited the anchorage-independent growth of the PC-3 cells,and induced their anoikis,all in a dose-and time-dependent manner（r = 0.935,P = 0.001;r = 0.901,P = 0.0005;r = 0.916,P = 0.0003）.Conclusion： MTA1 siRNA can inhibit the anchorage-independent growth of prostate cancer cells by inducing their anoikis.