硫化氢对大鼠肝缺血再灌注损伤的影响

目的 评价硫化氢对大鼠肝缺血再灌注损伤的影响.方法 健康雄性SD大鼠30只,体重220～250 g,采用随机数字表法,将其随机分为假手术组(S组)、缺血再灌注组(IR组)和不同剂量硫化氢组(H2S1～3组),每组6只.S组仅暴露肝门,不夹闭动、静脉；IR组采用夹闭左、中叶肝蒂、门静脉和肝动脉支1h恢复灌注的方法制备大鼠肝缺血再灌注模型；H2S1～3组于再灌注前5min分别腹腔注射14、28、56 μmol/kg硫化氢钠.于再灌注6h时抽取下腔静脉血样并取肝组织,采用全自动生化分析仪测定血清谷丙转氨酶(ALT)和谷草转氨酶(AST)活性,采用二硫代二硝基苯甲酸法测定肝组织谷胱甘肽(GSH)含量,光镜下观察肝组织病理学结果.结果 与S组相比,IR组血清ALT和AST活性升高,肝组织GSH含量下降(P＜0.05)；与IR组相比,H2S1～3组ALT和AST活性降低,肝组织GSH含量升高(P＜0.05)；H2S1～3组肝病理学损伤较IR组明显减轻.结论 H2S可减轻大鼠肝缺血再灌注损伤.

Effects of hydrogen sulfide on hepatic ischemia-reperfusion injury in rats

Objective To investigate effects of hydrogen sulfide on hepatic ischemia-reperfusion injury in rats.Methods Thirty healthy male SD rats weighing 220-250 g were randomly divided into sham operation group (S group),ischemia-reperfusion group(IR group) and different doses of hydrogen sulfide groups (H2S1～3 groups),every group contains six rats.In S group hepatic portal was only exposed but not ligated.In IR group the left and middle lobes of hepatic pedicle,portal vein and hepatic artery were clamped for 1 h and then reperfuse.H2 S1～3groups received intraperitoneal injection of NaHS 14,28,56 μmol/kg at 5 min before reperfusion.Blood samples were collected for measuring the activity of alanine transaminase (ALT) and aspartate transaminase (AST) in serum at 6 h after reperfusion.Liver tissue samples were collected for measurement the glutathione (GSH) content and pathologic examination.Results Compared with S group,the activity of ALT and AST in serum was increased,GSH content in liver tissue decreased in IR group ; Compared with IR group,the activity of ALT and AST in serum was decreased,GSH content in liver tissue increased in H2 S1～3 groups.Hepatic tissue pathologic injury was reduced in H2 S1～3 groups as compared with IR group.Conclusion Hydrogen sulfide can reduce hepatic ischemia-reperfusion injury in rats.