脓毒血症对大鼠罗库溴铵药效学的影响

目的 评价脓毒血症对大鼠罗库溴铵药效学的影响.方法 健康雄性SD大鼠40只,体重250～350 g,采用随机数字表法,将其随机分为对照组(C组,n=10)、假手术组(S组,n=10)和脓毒血症组(Sep组,n=20).Sep组采用盲肠结扎穿孔术制备脓毒血症模型,并于术后6和16 h时分别取10只大鼠,静脉注射罗库溴铵3.81 mg/kg;S组不结扎穿孔盲肠,仅于术后6h静脉注射罗库溴铵3.81 mg/kg.采用RM6240B型多道生理信号采集处理系统记录肌松起效时间、TOF无反应期、高峰时间、临床肌松作用时间、体内肌松作用时间、T1从最大抑制到恢复至10％、25％、50％、75％、90％的时间和恢复指数.结果与C组和S组比较,Sep组罗库溴铵肌松起效时间延长,TOF无反应期、高峰时间、临床肌松作用时间、体内肌松作用时间、T1从最大抑制到恢复至10％、25％、50％、75％、90％的时间和恢复指数缩短(P＜0.05)；Sep组术后16 h时注射罗库溴铵较术后6h时注射肌松起效时间延长,T1恢复至75％的时间缩短(P＜0.05).结论 脓毒血症可降低罗库溴铵的肌松效应,其效应与脓毒血症严重程度有关.

Effects of sepsis on pharmacodynamics of rocuronium in rats

Objective To evaluate the effects of sepsis on pharmacodynamics of rocuronium in rats.Methods Forty SD male rats weighing 250-350 g were randomly divided into 3 groups: control group(group C,n =10) ; sham operation group(group S,n =10)and sepsis group (group Sep,n =20).Cerum was ligated and perforated to produce sepsis model in Sep group,rocuronium 3.81 mg/kg was intravenously injected at 6 or 16 h after operation,each time contains 10 rats.Cecum was not ligate and perforate in group S,but rocuronium 3.81 mg/kg was intravenously injected at 6 h after operation.Onset time,TOF no reaction period,duration of peak effect,clinical duration,totel duration,time for recovery of T1 to 10％,25％,50％,75％,90％ and recovery index were recorded by RM6240B signal acquisition and processing system.Results Compared with groups C and S,onset time was significantly prolonged,TOF no reaction period,duration of peak effect,clinical duration,total duration and time for recovery T1 to 10％,25％,50％,75％,90％ and recovery index were shortened in group Sep ( P ＜ 0.05).Onset time was significantly prolonged,time for recovery of T1 to 75％ was shortened when rocuronium injection at 16 h after operation as compared with that at 6 h after operation in group Sep( P ＜ 0.05).Conclusion Sepsis can attenuate the effects of nondepolarizing neuromuscular blocker rocuronium,the degree is related to the stage of sepsis.