肝细胞癌中u-PA和MMP-9的表达及意义

目的探讨尿激酶型纤溶酶原激活物(u-PA)、基质金属蛋白酶-9(MMP-9)在肝细胞癌中的表达及其临床意义。方法采用免疫组化法对80例肝细胞癌标本进行u-PA和MMP-9检测,并以12例肝移植供肝作为阴性对照。结果u-PA及MMP-9在肝癌中的表达阳性率分别为83.75%、76.25%,明显高于癌旁组织(33.75%、30%),P<0.01。u-PA及MMP-9在伴转移及癌栓形成肝癌的阳性表达率分别为91.43%、85.71%,明显高于非转移者(77.78%、68.89%),P<0.05;且u-PA、MMP-9阳性表达间呈正相关。结论u-PA和MMP-9的高表达与肝细胞癌的侵袭转移有关,二者联检有助于判断肝细胞癌的预后。

Expression and clinical significance of u-PA and MMP-9 in hepatocellular carcinoma

[Objective] To investigate the protein expression and clinical orrelation of u-PA and MMP-9 in hepatocellular carcinoma.[Methods] The protein expression of u-PA and MMP-9 in 80 cases of hepatocellular carcinoma(HCC)and 12 cases from donators after transplanting operation was detected by immunohistochemistry.[Results] Immunohistochemistry revealed that among 80 cases,the positive rate of u-PA and MMP-9 in HCC was 83.75%,76.25% respectively,which was higher than that in paraneoplatic liver tissue(33.75%,30%),P< 0.01. And the u-PA and MMP-9 positive rate in HCC with metastasis(91.43%,85.71%) was also higher than that without metastasis (77.78%,68.89%),P< 0.05.There was a positive correlation between the positive expression of u-PA and MMP-9 in hepatocellular carcinoma.[Conclusion] The high expression of u-PA and MMP-9 in HCC may be related to the growth, invasion and metastasis of HCC,and they may be used as markers of prognosis of HCC in clinical practice.