体外培养碱性成纤维细胞因子对人牙周膜成纤维细胞生物学特性的影响

目的：探讨体外培养过程中，碱性成纤维细胞生长因子（bFGF）对人牙周膜成纤维细胞（hPDLFs）生物学特性的影响。方法：体外分离培养人牙周膜成纤维细胞并鉴定，加入不同浓度bFGF（1ng／ml、10ng／ml、50ng／ml、100ng／ml）培养，MTT方法检测细胞增殖情况；并对细胞进行矿化诱导，检测细胞的碱性磷酸酶活性。结果：hPDLFs呈星形或长梭形，免疫组化波丝蛋白阳性，角蛋白阴性，证实该细胞来源可靠。bFGF在一定浓度范围内，与细胞增殖成正比，而在本实验培养条件下（10％FBS）bFGF最大效应浓度为10ng／ml。矿化诱导条件下，碱性磷酸酶活性明显增加，在联合应用bFGF的情况下，100ng／ml组碱性磷酸酶活性明显高于其他组。结论：不同浓度bFGF对人牙周膜成纤维细胞生物学行为的影响不同，在一定浓度条件下，低浓度bFGF促进人牙周膜成纤维细胞的增殖，而高浓度bFGF作用于人牙周膜成纤维细胞可能更易于分化。

Effects of basic fibroblast growth factor on human periodontal ligament fibroblasts characteristics in vitro

Objective:To evaluate the effects of basic fibroblast growth factor on the characteristics of human periodontal ligament fibroblast in vitro.Method:Isolated human periodontal ligament fibroblasts(PDLFs) in vitro.The isolated cells were cultured in DMEM medium(10?S) and identified by immuno histochemistry.The cells cultured in different media with different dose of bFGF(1 ng/ml,10 ng/ml,50 ng/ml,100 ng/ml).And they were induced by mineralization induce liquor.The change of cells characteristics were observed by inverted phase contrast microscope,MTT and the alkaline phosphates level of cells.Result:The isolated cells were positive stain for vimentin and negative for cytokeratin.The results of MTT showed that there was a positive correlation between hPDLFs and the dose of bFGF in some defined condition.And the medium with 10 ng/ml bFGF was the optional medium for the proliferation of hPDLFs in vitro.Then the medium with 100 ng/ml bFGF was a proper medium for differentiation of hPDLFs in vitro.Conclusion:The results revealed that the effects of bFGF on hPDLFs were different during a period of time under specified condition.