硫化氢抑制前列腺癌骨转移PC-3细胞增殖和侵袭的体外研究

目的探讨硫化氢对前列腺癌骨转移PC-3细胞增殖和侵袭的影响及其可能机制。方法分别用0、1、2、4 mmol/LNaHS（硫化氢载体）作用前列腺癌骨转移PC-3细胞36 h,CCK-8比色法法检测细胞存活率,transwell法检测细胞侵袭能力,ELISA法检测细胞MMP-2和MMP-9分泌能力,Western-Blot法检测Bcl-2、Bax蛋白的表达。结果硫化氢对PC-3细胞生长的影响：用硫化氢孵育PC-3细胞36 h,1.0 mmol/L NaHS组细胞存活率与对照组比较,差异无统计学意义（P〉0.05）;2、4 mmol/L NaHS作用PC-3细胞36 h,细胞存活率降低,与对照组比较,能不同程度抑制PC-3细胞的生长,差异有统计学意义（P〈0.05或P〈0.01）。硫化氢对PC-3细胞侵袭的影响：用硫化氢孵育PC-3细胞36 h,1 mmol/L NaHS组细胞侵袭数与对照组比较,差异无统计学意义（P〉0.05）;2、4 mmol/L NaHS作用PC-3细胞36 h,细胞侵袭数减少,与对照组比较,能不同程度抑制PC-3细胞的侵袭,差异有统计学意义（P〈0.05或P〈0.01）。ELISA法检测PC-3细胞MMP-2和MMP-9分泌能力：用硫化氢孵育PC-3细胞36 h,1 mmol/L NaHS组细胞MMP-2和MMP-9分泌量与对照组比较,差异无统计学意义（P〉0.05）;2、4 mmol/L NaHS作用PC-3细胞36 h,MMP-2和MMP-9分泌量减少,与对照组比较,能不同程度抑制PC-3细胞MMP-2和MMP-9的分泌,差异有统计学意义（P〈0.05或P〈0.01）。Western blot检测PC-3细胞Bcl-2/Bax蛋白表达：1、2、4 mmol/L NaHS组,Bcl-2表达降低,Bax表达升高,呈浓度依赖性。结论 2 mmol/L浓度以上的NaHS能影响Bcl-2和Bax表达和MMP-2 MMP-9分泌来抑制PC-3细胞的生长和侵袭。

Invitrostudy of hydrogen sulfide inhibits proliferation and invasion of prostate cancer bone metastasis PC-3 cells

Objective To investigate the effect of hydrogen sulfide on prostate cancer bone metastasis PC-3 cells pro liferation and invasion in vitro and the possible mechanism.Methods PC-3 cells were treated with NaHS（a donor of hydrogen sulfide） at 0,1,2,4 mmol/L concentration for 36 h,respectively,then cell viability was tested by cell counter kit-8.Transwell assay were used to detect PC-3 cells invasive ability and ELISA was used to detect MMP-2 and MMP-9 secretion.PC-3 cells were treated with NaHS at 0,1,2,4 mmol/L concentration for 36 h,then the ex pressions of Bcl-2,Bax were evaluated by Western-Blot assay.Results The effects of hydrogen sulfide on PC-3 cells proliferation： the differences of cell viabilitie were not significant（P 0.05） when PC-3 cells were treated with NaHS at 1 mmol/L concentrations.When PC-3 cells were treated with NaHS at 2,4 mmol/L concentration,the cell viability was declined,compare with control group（P 0.05,P 0.01）.The effects of hydrogen sulfide on PC-3 cells invasive： the numbers of transfer to the other side of transwell membrane were counted in tumor invasive assay.The difference of 1 mmol/L NaHS group was not significant compared with control group（P 0.05） and 2 mmol/L NaHS group and 4 mmol/L NaHS group were declined compared with control group（P 0.05 or P 0.01）.The effect of hydrogen sulfide on MMP-2 and MMP-9 secretion： when cells were treated by 1 mmol/L NaHS,the secretion of MMP-2 and MMP-9 was not significant compared with control group（P 0.05）,2 mmol/L NaHS group and 4 mmol/L NaHS group were declined of MMP-2 and MMP-9 secretion compared with control group（P 0.05 or P 0.01）.The effects of hydrogen sulfide on PC-3 cells Bcl-2 and Bax expression： the expression of Bcl-2 was declined and Bax expression in creased compare with control group.Conclusion Hydrogen sulfide can inhibit the growth and invasive of PC-3 cells at the concentration 2,4 mmol/L by affecting Bcl-2 and Bax expression and reducing secretion of MMP-2 and MMP-9.