早期糖尿病大鼠视网膜毛细血管细胞凋亡及其特征

目的：研究早期糖尿病大鼠视网膜毛细血管细胞凋亡及其特征。方法：腹腔内注射链脲佐菌素诱导40只糖尿病大鼠模型,随机分为正常对照组（CON）、糖尿病1月组（DM1）、3月组（DM3）、6月组（DM6）。应用PAS染色、末端脱氧核苷酸转移酶介导的dUTP缺口标记（TUNEL）法标记和染射电镜观察进行研究。结果：于糖尿病3月和6月组,见周细胞和内皮细胞鬼影。周细胞和内皮细胞核异染色质聚集靠边,并随病程进展而加重。被TUNEL法标记的细胞核染色质分布不均,表现为环形核、新月形核等。结论：早期糖尿病大鼠视网膜毛细血管周细胞丧失的性质为细胞凋亡,内皮细胞亦存在调亡。

A study on the apoptosis of capillary cells in early diabetic rats

Objective:To investigate the apoptosis of retinal capillary cells in early diabetic retinopathy (DR) and observe apoptotic features.Methods:Diabetes was induced in rats with a single injection of streptozotocin (STZ) given intraperitoneally. Specimens were then obtained by using PAS staining, terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) methods as well as transmission electron microscopy.Results:"Ghosts" of endothelial cells and pericytes were seen in diabetes mellitus 3 months and 6 months after disease onset. The metachromatin of pericyte and endothelial cells could be observed to cluster to the side. This change became more severe with the progression of diabetes. The chromatin of nuclei labeled by the TUNEL method is distributed unequally, displaying apoptotic characteristics of ring like and crescent nuclei.Conclusion:Pericyte dropout in early DR in rats is due to apoptosis and apoptosis is also found in endothelial cells.