8株霍乱弧菌耐药状况及RAPD同源性分析

目的分析蚌埠市2010年霍乱弧菌对抗生素的敏感性和同源性,为霍乱防控提高依据。方法选取2010年安徽省蚌埠市O1/O139群霍乱病例分离株8株,采用WHO推荐的改良Kirby—Bauer纸片法,分析霍乱弧菌对15种抗生素在体外的药物敏感性;应用随机引物扩增多态性(RAPD)技术对其作分子流行病学分型和同源性分析。结果所有O1/O139群霍乱弧菌对氨苄西林、头孢唑啉、丁胺卡那、庆大霉素、舒普生、氧哌嗪青霉素、头孢曲松、亚胺培南均100%敏感,对复方新诺明、四环素、强力霉素均100%耐药。O139群霍乱弧菌在氯霉素、诺氟沙星、环丙沙星等的敏感度上与O1群霍乱弧菌有一定的差异。根据随机扩增多态DNA聚类分析可以将检出的O1/O139群霍乱弧菌分为2个型别。结论 O139群霍乱弧菌耐药率较O1群霍乱弧菌高,应根据不同菌型选择相应的抗生素药物进行治疗。分离的O1群霍乱弧菌有同源性。

Analysis On Drug-resistant State And RAPD Identity From 8 Strains Of Vibrio Cholerae

Objective To analyze the sensitivity and the identity of vibrio cholerae to antibiotics in Bengbu in 2010,and provide evidence for cholera control.Methods 8 strains from cholera o1/o139 cases in Bengbu city in 2010 were selected and used to analyze the drug sensitivities of vibrio cholerae to 15 antibiotics in vitro by adopting improved Kirby-Bauer paper method which were recommended by WHO,and their epidemiological pattern and identity were analyzed by random amplified polymorphic DNA（RAPD） technique.Results All of vibrio cholerae o1/o139 strains were sensitive to ampicillin,cefazolin,amikacin,gentamicin,sulperazon,piperacillin,ceftriaxone and imipenem,and were resistant to sulfamethoxazolum-trimethoprimum,tetracycline and doxcycline.So far as chloramphenicol,norfloxacin,ciprofloxacin,and so on,the sensitivity of vibrio cholerae o139 had some difference with vibrio cholerae o1.vibrio cholerae o1/o139 could be divided into two types according to the cluster analysis of RAPD.Conclusion The drug resistance rate of vibrio cholerae o139 was higher than one of vibrio cholerae o1.The treatment for different bacterial types should select corresponding antibiotics.Vibrio cholerae o1 strains isolated had the dentity.