| 桑菊饮含药血清对小鼠巨噬细胞Toll样受体表达的影响 |
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| 引用本文： | 杜新亮,隋 峰,张畅斌,赵保胜,刘洪斌,闫美娟,李兰芳,郭淑英,霍海如,姜廷良.桑菊饮含药血清对小鼠巨噬细胞Toll样受体表达的影响[J].中国实验方剂学杂志,2010,16(1):57-61. |
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| 作者姓名： | 杜新亮 隋 峰 张畅斌 赵保胜 刘洪斌 闫美娟 李兰芳 郭淑英 霍海如 姜廷良 |
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| 作者单位： | 中国中医科学院中药研究所,唐氏中药研究中心,北京,100700 |
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| 基金项目： | 教育部科学技术研究项目 |
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| 摘 要： | 目的: 研究桑菊饮对小鼠肺巨噬细胞株RAW264.7表达Toll样受体(TLR)的影响。 方法: 用桑菊饮含药血清作用于正常RAW264.7细胞,24 h后以荧光定量PCR方法测定TLR-1~TLR-9 mRNA的表达,探讨桑菊饮对Toll样受体表达的影响。结果:桑菊饮含药血清在一定剂量(7.7 g·kg-1·U-1)时,可以促进TLR-4和TLR-7的表达,而对TLR-1,TLR-2,TLR-3,TLR-5,TLR-6,TLR-8和TLR-9的表达没有明显影响。 结论: 桑菊饮的药效学作用可能与促进Toll样受体某些亚型表达有关。
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| 关 键 词： | 桑菊饮 含药血清 肺巨噬细胞 Toll样受体7 Toll样受体4 |
| 收稿时间： | 2009/4/16 0:00:00 |
Effect of the Drug Serum Containing Sangjuyin on the Expression of Toll-like Receptors in RAW 264.7 Cells |
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| DU Xin-liang,SUI Feng,ZHANG Chang-bin,ZHAO Bao-sheng,LIU Hong-bin,YAN Mei-juan,LI Lan-fang,GUO Shu-ying,HUO Hai-ru and JIANG Ting-liang.Effect of the Drug Serum Containing Sangjuyin on the Expression of Toll-like Receptors in RAW 264.7 Cells[J].China Journal of Experimental Traditional Medical Formulae,2010,16(1):57-61. |
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| Authors: | DU Xin-liang SUI Feng ZHANG Chang-bin ZHAO Bao-sheng LIU Hong-bin YAN Mei-juan LI Lan-fang GUO Shu-ying HUO Hai-ru and JIANG Ting-liang |
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| Institution: | Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
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| Abstract: | Objective: To investigate the influence of Sangjuyin drug serum on the expression of TLRs on RAW264.7. Methods: RAW264.7 cell line was stimulated with the drug serum containing Sangjuyin. Expressions of TLRs mRNA were detected with real-time PCR after 24 hours. Results: The serum contained Sangjuyin could upregulated the expression of TLR-3 mRNA and TLR-7 mRNA in RAW264.7 cells. Conclusion: Promoting the expression of Toll-like receptors mRNA may be the elementary basis of Sangjuyin to play the effect. |
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| Keywords: | Sangjuyin drug serum pulmonary macrophage TLR-7 TLR-4 |
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