Flip and Flop Variants of AMPA Receptors in the Rat Lumbar Spinal Cord |
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Authors: | T. R. Tö lle,A. Berthele,W. Zieglgä nsberger,P. H. Seeburg,W. Wisden |
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Affiliation: | Max Planck Institute of Psychiatry, Clinical Institute, Clinical Neuropharmacology, Kraepelinstrasse 10, 80804 Munich, Germany;Centre for Molecular Biology (ZMBH), University of Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany |
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Abstract: | The expression of eight messenger RNA splice forms encoding the Flip and Flop variants of AMPA receptor subunits GluR-A to -D in the rat lumbar spinal cord was examined by in situ hybridization using specific oligonucleotides. In the dorsal horn (laminae I, II and III) the predominant mRNA was GluR-B Flip. Much lower levels of GluR-A Flip were found in lamina I and in superficial parts of lamina II outer. In the ventral horn, motor neurons expressed mainly GluR-B Flip, GluR-C Flip and Flop, and GluR-D Flip. Serial sectioning through large motor neurons indicated that a given cell contained, for example, both GluR-C Flip and Flop splice types. |
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Keywords: | splice variants dorsal horn motor neuron in situ hybridization |
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