白介素—1受体相关激酶—2反义寡核苷酸对白介素—1和肿瘤坏 …

目的 研究白介素－１受体相关激酶０２（ＩＲＡＫ－２）在白介素－１（ＩＬ－１）与肿瘤坏死因子（ＴＮＦ）激活核因子０ｋＢ（ＮＦｋＢ）过程中的作用。方法 ＩＲＡＫ－２反义胡核苷酸转染人胚在子刺激细胞后,用夹心ＥＬＩＳＡ方法检测ＮＦ－ｋＢ活性。结果以ＩＢＡＫ－２反义寡核苷酸预处理可明显减弱ＩＬ－１诱导的ＮＦ－ｋＢ活性,此效应强度存在时间和浓度依赖性,但ＩＲＡＫ－２反义;寡核苷酸对肿瘤坏死因子话导的ＮＦ－

Different responses of IL-1-and TNF-stimulated NF-

AIM　To investigate the role of interleukin-1 ( IL-1 ) receptor associated kinase-2 (IRAK-2) in IL-1-and TNF-stimulated nuclear factor-kappa B ( NF-κB ) activation.METHODS　The 293 cell was transfected with antisense IRAK-2 oligonucleotide (IRAK-2 ODN) followed by stimulating the cell with IL-1 or tumor necrosis factor (TNF), and then the levels of NF-κB activation was analyzed by sandwich enzyme-linked immunosorbent assay (ELISA). RESULTS　Pre-transfecting with antisense IRAK-2 ODN could remarkably decrease the levels of NF-κB activation stimulated by IL-1 in time and concentration-dependent manner,and IRAK-2ODN not attenuate the one stimulated by TNF. CONCLUSION　The responses of IL-1-and TNF-stimulated NF-κB activation to antisense IRAK-2 oligonucleotids are different. IRAK-2 plays a key role in the IL-1 signaling events leading to NF-κB activation.