酸性胃蛋白酶法收集猪带绦虫虫卵的实验研究

目的　建立收集绦虫卵的新方法。　方法　用 1%酸性胃蛋白酶溶液消化猪带绦虫孕节 ,观察不同条件下消化孕节收集虫卵的时间 ;比较酸性胃蛋白酶法与物理法收集的虫卵数量及其孵化后六钩蚴存活率 ;观察 1%酸性胃蛋白酶消化孕节 40min和 12 0min收集的虫卵以次氯酸钠法孵化后虫卵孵化率及六钩蚴存活率。　结果　 1%酸性胃蛋白酶振荡条件下消化孕节收集虫卵时间短于普通条件下的时间 (P <0 .0 1) ,消化剪碎孕节时间短于消化完整孕节时间 (P<0 .0 1) ;酸性胃蛋白酶法收集虫卵的数量较物理法提高 6.975 % ;酸性胃蛋白酶法和物理法收集的虫卵以次氯酸钠法孵化后孵化率及六钩蚴存活率差异无显著性 (P >0 .0 5 ) ;1%酸性胃蛋白酶消化孕节 40min和 12 0min收集的虫卵以次氯酸钠法孵化后孵化率及六钩蚴存活率差异无显著性 (P >0 .0 5 )。　结论　成功建立了较为适用的新的猪带绦虫虫卵收集方法—酸性胃蛋白酶法。

STUDIES ON THE METHOD OF COLLECTING EGGS OF TAENIA SOLIUM BY THE ACIDITY PEPSIN

Objective To establish a new method for collecting eggs of Taenia solium . Methods The integrity and smashed gravid proglottids of T. solium were digested by pepsin solution in different condition. The time of digesting gravid proglottids was observed meanwhile;The number of eggs collected by physical method and pepsin method was compared;The hatching rate of eggs and the viability of oncospheres were observed after the eggs collected by two methods as above,and after the gravid proglottids were digested in 40 min or 120 min by pepsin solution. Sodium hypochlorite method was used in egg hatching. Results The time of gravid proglottids digested by pepsin solution in surge condition was less than in common condition( P <0.01),and the time of the integrity gravid proglottids digested by pepsin solution was less than that of smashed( P <0.01);The number of eggs collected by pepsin method was 6.975% more than that collected by physical method;The hatching rate of eggs and the viability of oncospheres after the eggs collected by physical method or pepsin method were not different( P >0.05);The hatching rate of eggs and the viability of oncospheres after the gravid proglottids were digested 40 min or 120 min by pepsin solution were also not different( P >0.05). Conclusion A new method for collecting eggs of T. solium by digesting gravid proglottids by the acidity pepsin was established.