水蛭抗凝血活性成分的研究

目的：研究水蛭的抗凝血成分。方法：以血浆复钙时间为指标，应用Sephadex DEAE A-50阴离子交换树脂色谱、Sephadex G-25和Sephadex LH-20凝胶过滤色谱及反相高压液相色谱技术分离纯化。结果：得到3个抗凝血活性多肽，其中化合物1，2在自然条件下互相转化，它们的相对分子质量分别为7 100和5 531；采用HPLC及SDS-PAGE鉴定了化合物3的纯度，采用MALDI-TOF-MS法测定出其相对分子质量为8 608，并测定了其氨基酸组成。结论：推测化合物3为首次分离得到的抗凝血多肽，命名为蚂蟥多肽(whitmanin)。

Studies on anticoagulant constituents in dried Whitmania pigra

Objective: To study the anticoagulant constituents in dried leech (Whitmania pigra Whitman). Method: The plasma recalcification time (PRT) as index, the constituents with anticoagulant activity were isolated and purified by anion-exchange chromatography on Sephadex DEAE A-50, gel permeation chromatography on Sephadex G-25 and Sephadex LH-20 columns, and then reversed phase high-performance liquid chromatography successively. Result: Three anticoagulant polypeptides were isolated and purified. Compounds 1 and 2 can be translated each other in natural conditions, and their molecular weights are 7 100 and 5 531, respectively. Compound 3 was identified as a pure polypeptide by HPLC and SDS-PAGE, and its molecular weight was determined as 8 608 by MALDI-TOF-MS. The amino acid composition of compound 3 was also determined. Conclusion: Compound 3 was inferred to be a novel anticoagulant, and named whitmanin.