芹菜素对小鼠精液参数影响的研究

目的:探讨芹菜素对小鼠精液参数的影响。方法:成年健康SPF级昆明种雄性小鼠100只按体重随机分成5个剂量组(阴性对照组、溶剂对照组及芹菜素低、中和高剂量组),各剂量组又随机分成7 d和14 d处理组。每天定时依次灌胃,7 d处理组和14 d处理组分别连续灌胃7 d和14 d,在首次灌胃第35 d时处死小鼠,取材,应用计算机辅助精液分析技术(CASA)检测小鼠精子运动参数。结果:芹菜素7 d处理各剂量组精子运动参数及精子密度、a+b级精子百分率与阴性对照组比较差异无统计学意义;芹菜素14 d处理高剂量组平均路径速度(VAP)、直线速度(VSL)、前向性(STR)、摆动性(WOB)、b级精子所占比例及a+b级精子百分率低于阴性对照组,鞭打频率(BCF)高于阴性对照组,均有统计学意义(P<0.05)。结论:芹菜素对小鼠精子运动参数有一定的影响。

Apigenin affects semen parameters in male mice

Objective:To explore the effect of apigenin on semen parameters in male mice.Methods:Totally 100 healthy male mice of Kunming strain were randomly divided into 5 groups according to the body weight:negative control,solvent control,low-dose apigenin,median-dose apigenin and high-dose apigenin,the latter three groups given intragastric apigenin at a fixed time every day for 7 and 14 days.At 35 days after the first medication,all the mice were killed and detected for the sperm motion parameters by computer aided sperm analysis(CASA).Results:There were no statistically significant differences in sperm motion parameters,density and motility between the negative control and the three apigenin groups after 7-day medication.At 14 days,the high-dose apigenin group showed remarkable decreases in average path velocity(VAP),straight line velocity(VSL),straightness(STR),wobbliness(WOB),the percentage of grade b sperm and sperm motility,and a significant increase in beat cross frequency(BCF) as compared with the negative control group(P < 0.05).Conclusion:Apigenin affects sperm motility in male mice to a certain extent.Natl J Androl,2010,16(9):778-782