大剂量甲基强的松龙对脊髓损伤后神经细胞凋亡的影响

目的　探讨大剂量甲基强的松龙对脊髓损伤后神经细胞凋亡的影响。　方法　将60只大鼠分为脊髓损伤后应用大剂量甲基强的松龙组(A组，30只)和单纯脊髓损伤组(B组，30只)。损伤后1，3，7，14，28　d，对脊髓损伤区进行细胞凋亡的检测（原位末端脱氧核糖核苷酸转移酶介导dUTP标记法，TUNEL）以及Bcl-2蛋白表达的测定（免疫组化SP法）。　结果　A、B两组中均发现凋亡细胞及Bcl-2蛋白阳性表达。大鼠脊髓损伤后3d图像分析表明，细胞凋亡率分别为A组（12.43±5.16），B组（36.27±6.28）；Bcl-2蛋白阳性细胞分别为A组（37.68±5.83），B组（21.48±7.83）。两组比较，差异有显著性意义(P<0.01和0.05)。　结论　脊髓损伤可导致神经细胞凋亡，大剂量甲基强的松龙能抑制细胞凋亡。

Effect of high dose methylprednisolone on cell apoptosis after spinal cord injury

Objective　 To investigate the effect of high dose methylp rednisolone(MP) on cell apoptosis after spinal cord injury (SCI). 　Methods　Sixty experimental rats were divided equally into 2 g roups, Allen's weight drop spinal cord injury and high dose methylprednisolo ne g roup (Group A) and Allen's weight drop injury group (Group B). The cell apoptosi s of spinal slice from the injured spinal cord was examined by the terminal deox ynucleotidal transferase-mediated DUTP-biotin nick end labeling (TUNEL) reacti on, and the expression of Bcl-2 measured immunohistochemically 1, 3, 7, 14 and 28 days post-operatively. The positive cells were quantitated by a computer ima ge analysis system. 　Results　 Positive expression of apoptotic cells and Bcl-2 pr otein appeared both in Group A and Group B. SCI for 3 days, the computer image a nalysis system showed the apoptosis rate was 12.43±5.16 in Group A and 36.27±6.28 in Group B; Bcl-2 protein expression positive cells were 37.68± 5.83 in Group A and 21.48±7.83 in Group B. There was a significant stat istic difference remained between Group A and Group B (P<0.01 and 0. 05). 　Conclusions　The high dose methylprednisolone can inhibit apop tosis after SCI.