RP-HPLC法同时测定大鼠血中甘草甜素和甘草次酸

目的　建立一个同时测定甘草甜素、甘草次酸血浆浓度变化监测的方法 ,进一步用于“脾主药动学”假说的验证 .方法　采用内标定量的反相高效液相色谱法 ,以甲醇 -水 -醋酸 (88∶ 11∶ 1)为流动相 ,ODS- 3柱 (15 0 mm× 4.6 mm,5μm)为固定相 ,在紫外 2 5 0 nm检测 .结果　 GL,GA在此线性范围内 ,线性关系良好、精密度较高 .结论　本方法具有简便、灵敏、快速而准确的特点 ,适用于注射 GL制剂或口服中药复方前后 GL和 GA的体内、体外的药代动力学研究 ,为进一步进行中药复方的药物监测奠定了基础

Simultaneous measurement of glycyrrhizin and glycyrrhetinic acid in rat serum by RP-HPLC method

AIM　Establishment of method for measuring plasma concentration of the glycyrrhizin and glycyrrhetinic acid simultaneously by RP-HPLC, in order to testify the hy-pothesis of pharmacokinetics by traditional Chinese medicine. METHODS　RP-HPLC with internal labeling was employed. Methanol-H2O-acetic acid (88∶11∶1) was used as mobile phase and ODS-3 (150 mm×4.6 mm, 5 μm) column as fixed phase, and detected by UV (λ=250 nm). RESULTS　Within the linear range the glycyrrhizin and glycyrrhetinic acid showed good linear relation and were detected with high precision.CONCLUSION　Method is characterized by high simplicity, rapidity, and sensitivity, and is suitable for the in vitro and in vivo pharmacokinetic study of glycyrrhizin and glycyrrhetinic acid after oral administration or injection of GL extracts.