A rapid and efficient method for the purification of the complement subcomponents C1r and C1s in zymogen form using fast protein chromatography |
| |
| Authors: | M C Peitsch T J Kovacsovics H Isliker |
| |
| Institution: | Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland. |
| |
| Abstract: | The purification of the subcomponents C1r and C1s of the first component of complement involves multiple steps and is time-consuming. This accounts for the frequently observed partial activation of the subcomponents. In this report we propose a simplified procedure of purification using a batch method and fast protein chromatography avoiding a shift of pH. The method provides C1r and C1s in a yield of 35 and 60% respectively. In addition, this study provides a simple and sensitive test to assess functional purity of C1r and C1s with respect to the other C1 subcomponents. |
| |
| Keywords: | |
|
|
