胃黏膜肠化及胃癌中三叶因子1,2表达的组织芯片技术研究

目的:三叶因子1(trefoil factor 1,TFF1)和2(TFF2)对胃肠道黏膜保护和损伤后修复具有重要作用,在胃癌中表达降低.现探讨它们在胃黏膜肠化(intestinal metaplasia,IM)及不同组织类型胃癌发生中的作用.方法:胃癌40例,癌旁组织IM 32例,慢性萎缩性胃炎(chronic atrophic gastritis,CAG)伴IM 46例,CAG和慢性浅表性胃炎(chronic superficial gastritis,CSG)各20例,构建组织芯片.分别用高铁二铵/爱先蓝(HID/AB)及HE染色对IM及胃癌进行分型,并用免疫组化检测不同胃黏膜病变中TFFl,TFF2的表达.结果:TFFl,TFF2在不同胃黏膜病变中的表达有显著性差异(P＜0.01),CSG,CAG中的表达显著高于CAG伴IM、癌旁组织IM及胃癌(P＜0.01),而CSG与CAG之间,以及CAG伴IM、癌旁组织IM及胃癌三者之间比较均无显著性差异(P＞0.05).肠型胃癌则显著低于弥漫型胃癌(P＜0.05).结论:胃黏膜IM及胃癌中TFF1,TFF2表达显著降低.

Analysis of expression of trefoil factor 1 and 2 in gastric intestinal metaplasia and gastric cancer by tissue microarray

AIM: To investigate roles of trefoil factor 1 (TFF1) and trefoil factor 2 (TFF2) in intestinal metaplasia (IM) and gastric carcinogenesis. METHODS: Forty gastric cancers, 32 IM foci in corresponding paracancerous tissues, 46 IM foci in chronic atrophic gastritis (CAG), 20 CAG, and 20 chronic superficial gastritis (CSG) were selected respectively to construct tissue microarrays. High iron diamine/alcian blue (HID/AB) staining and HE staining were used to classify the IM and gastric cancer and the expressions of TFF1 and TFF2 protein were assessed with immunohistochemistry. RESULTS: The expressions of TFF1 and TFF2 in different gastric lesions were significantly different (P<0.01), significantly higher in CSG and CAG than in IM foci in CAG, IM foci in paracancerous tissues, and gastric cancer (P<0.01). But no significant difference was found between CSG and CAG, as well as among IM foci in CAG, IM foci in paracancerous tissues, and gastric cancer (P<0.05). The expressions of TFF1 and TFF2 in the intestinal-type gastric cancer were both lower than those in the diffuse-type (P<0.05). CONCLUSION: The expressions of TFF1 and TFF2 significantly decrease in gastric IM and gastric cancer.