锰超氧化物歧化酶功能基因多态性及酶活性与迟发性运动障碍的关系

目的探讨锰超氧化物歧化酶 (MnSOD)功能基因Ala 9Val多态性及血浆MnSOD活性与抗精神病药所致迟发性运动障碍 (TD)的关系。方法采用Schooler和Kane的TD研究诊断标准和异常不自主运动量表 (AIMS) ,对 42例伴有TD(TD组 )和 5 9例不伴有TD(非TD组 )的长期使用抗精神病药物治疗的男性精神分裂症患者 ,以及 5 0名健康男性 (对照组 ) ,应用聚合酶链反应及限制性片段长度多态性技术分析MnSOD基因Ala 9Val多态性的等位基因和基因型分布 ,同时采用黄嘌氧化酶法测定血浆中Mn SOD活性。结果MnSOD基因Ala 9Val多态性的等位基因和基因型分布在患者组 (n =10 1)与对照组之间、以及TD组与非TD组及对照组之间差异均无显著性 (P均 >0 .0 5 ) ,TD组血浆MnSOD活性 [(10 2±73 )NU/ml]明显高于非TD组 [(5 0± 5 5 )NU/ml]及对照组 [(5 8± 3 8)NU/ml] (P均 <0 .0 5 ) ,TD组中不同基因型患者血浆MnSOD活性差异无显著性 (P >0 .0 5 )。结论抗精神病药所致迟发性运动障碍可能与血浆MnSOD活性增高有关 ,但可能与MnSOD功能基因Ala 9Val多态性无关联

Relationship of manganese superoxide dismutase gene polymorphism and the enzyme activity in plasma with tardive dyskinesia in schizophrenia

ObjectiveTo investigate whether the association of Ala-9Val functional polymorphism of manganese superoxide dismutase (MnSOD) gene and the MnSOD activity in plasma with antipsychotic-induced tardive dyskinesia (TD) in schizophrenia.Methods Diagnosis of TD was assessed by the Abnormal Involuntary Movement Scale (AIMS).The polymorphism of the MnSOD gene was analysed using a polymerase chain reaction (PCR)-based restriction fragment length polymorphism (RFLP) method.MnSOD activity in plasma was measured by using Xanthine oxidase method. ResultsThere were no significant differences in the distributions of the allelic frequencies and genotypes between schizophrenic patients (n=101) and healthy controls (n=50) (P> 0.05 ) ,and between the patients with TD (n=42) and without TD (n=59) and normal controls (P>0.05).The patients with TD showed a significantly higher level of MnSOD activity as compared to the patients without TD (P<0.05)and normal controls (P<0.05). Conclusions No evidence that the MnSOD gene Ala-9Val polymorphism is likely to confer susceptibility to the development of TD in Chinese male patients with schizophrenia. The high level of MnSOD activity in plasma may be a risk factor for the development of TD.