非小细胞肺癌中MMP-2、CD44V6的表达及其临床意义

目的 探讨细胞黏附因子（CD44V6）和基质金属蛋白酶-2（MMP-2）在非小细胞肺癌（NSCLC）组织中的表达及其与肺癌浸润、转移和预后的关系。方法 回顾性分析43例术前未进行放、化疗的肺癌患者的切除标本，采用免疫组化SP法检测肺癌组织中的CD44V6和MMP-2的表达。结果 肺鳞癌与肺腺癌中CD44V6的阳性表达率分别为58．33％（14／24）和68．42％（13／19）；MMP-2的阳性表达率分别为54．16％（13／24）和84．21％（16／19）。CD44V6与MMP-2的阳性表达与淋巴结转移、肺癌病理分期以及术后血行转移显著相关（P〈0．05），CD44V6阳性表达者的3年生存率为25．62％，5年生存率为6．41％；阴性表达者的3年生存率为64．71％，5年生存率为56．82％，两者差异显著（P=0．000）。MMP-2阳性者的3年生存率为18．8％，5年生存率为8．67％；阴性表达者的3年生存率为66．30％，5年生存率为42．66％，两者差异显著（P=0．0067）。CD44V6和MMP-2的阳性表达呈显著性相关（P：0．007）。结论CD44V6和MMP-2对于肺癌的侵袭、淋巴结转移、术后血行转移以及预后有一定作用。

Expression of CD44V6 and matrix metalloproteinase-2 in non-small cell lung cancer

Objective:To detect the expression of cell adhesion molecules-v6(CD44V6) and matrix mettalloproteinase-2(MMP-2) in non-small cell lung cancer(NSCLC),and to explore their association with invasion metastasis and prognosis.Methods:A rapid immunohistochemical method(streptoavidin-peroxidase SP) was used to detect CD44V6 and MMP-2 proteins expression in 43 cases of NSCLC,these cases were not treated radiotherapy or chemotherapy before operation.Results:The expression rates of CD44V6 in lung squamous cell carcinoma and lung adenocarcinoma were 58.33%,68.24% respectively.The positive expression rates of MMP-2 in lung squamous cell carcinoma and lung adenocarcinoma were 54.16%,84.21% respectively.The positive expression of MMP-2 and CD44V6 were significantly correlated with lympha node metastasis,TNM stage and postoperative hematogenous metastasis (P<0.05) The 3-year,5-year survival rates of CD44V6 positive groups were 25.62%,6.41%,negative expression groups were 64.71%,56.82%,The significant difference of survival time was displayed between CD44V6 positive and negative expression groups(P=0.000).The 3-year,5-year survival rates of MMP-2 positive expression were 18.8%,8.60%,negative expression groups were 66.30% and 42.82% separatately,The significant difference was showed between MMP-2 positive expression and negative expression groups(P=0.0067).The positive expression of CD44V6 was significantly correlated with MMP-2 positive expression(P=0.007).Conclusion:CD44V6 and MMP-2 may play a role in NSCLC invasion,lympha node metastasis,postoperative hematogenous metastasis and prognosis.