Protein A is a virulence factor in Staphylococcus aureus arthritis and septic death |
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| Authors: | Palmqvist Niklas Foster Timothy Tarkowski Andrzej Josefsson Elisabet |
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| Institution: | 1. Department of Rheumatology and Inflammation Research, Göteborg University, Guldhedsgatan 10A, S-413 46, Göteborg, Sweden;2. Department of Microbiology, Trinity College, Dublin, Ireland;3. Ohio State University, Columbus, Ohio 43210;4. Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, PE, 50.740-465, Brazil;5. Center for Infectious and Inflammatory Diseases, Institute of Biosciences and Technology, Texas A&M Health Science Center, Houston, Texas 77030;1. Uro-Oncology Research Center, Tehran University of Medical Sciences, Tehran, Iran;2. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran;1. Laboratory of Experimental and Molecular Immunology and Neurogenetics(INEM), UMR 7355 CNRS-University of Orleans, Orleans, France;2. Department of Pharmacology, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Brazil;1. Department of Pathology, Microbiology & Immunology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA;2. Graduate Program in Microbiology & Immunology, Vanderbilt University, Nashville, TN, 37232, USA;1. Department of Clinical Microbiology, Royal College of Surgeons in Ireland Education and Research Centre, Beaumont Hospital, Dublin, Ireland;2. Department of Microbiology, Beaumont Hospital, Dublin, Ireland;3. Microbiology Research Unit, Dublin Dental University Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland;1. Bacteriology, Department of Infectious Diseases, Faculty of Veterinary Medicine and Institute for Fundamental and Applied Research in Animal Health (FARAH), University of Liège, Sart-Tilman, Bât. 43a, B-4000 Liège, Belgium;2. Environment & Public Health Research Group, School of Environment & Technology, University of Brighton, Cockcroft Building, Lewes Road, Brighton BN2 4GJ, United Kingdom;3. Biostatistics, Bioinformatics and Animal Selection, Department of Animal Production, Faculty of Veterinary Medicine, University of Liège, Sart-Tilman, Bât. 43a, B-4000 Liège, Belgium;4. Agroscope, Institute for Food Sciences IFS, Schwarzenburgstrasse 161, 3003 Berne, Switzerland;5. Università degli Studi di Milano, Department of Health, Animal Science and Food Safety, via Celoria 10, 20133 Milano, Italy;6. Cornell University, Animal Health Diagnostic Center, Quality Milk Production Services, 240 Farrier Road, Ithaca, NY 14853, USA;7. Quantitative Genetics Group, Department of Animal Production, Faculty of Veterinary Medicine, University of Liège, Sart-Tilman, Bât. 43a, B-4000 Liège, Belgium |
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| Abstract: | Staphylococcal protein A (SpA), a cell wall anchored protein of Staphylococcus aureus, has the ability to interact with several host components, possibly indicating a role as a virulence factor in S. aureus infections. In this study, the contribution of SpA to bacterial virulence was investigated in a murine model of S. aureus arthritis. Intravenous inoculation of S. aureus wild-type strain Newman gave rise to more severe arthritis and higher mortality than the isogenic spa mutant strain DU5873. The wild-type strain caused more in vitro spleen cell proliferation than the SpA-deficient strain. However, IL-6 and TNF-alpha levels were higher after stimulation with thespa mutant strain compared to the wild-type strain. To conclude, our results clearly indicate that SpA is a virulence factor of S. aureus in murine septic arthritis. |
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