aP2增强子和启动子荧光素酶基因载体的构建及鉴定

目的构建aP2基因增强子和启动子驱动虫荧光素酶表达的真核表达载体，为进一步研究aP2基因表达调控机制及其信号转导通路等奠定基础。方法提取小鼠肝脏基因组DNA，PCR扩增aP2增强子和启动子，并将其与虫荧光素酶报告质粒PGL3-Basic连接，通过测序鉴定。结果小鼠aP2基因的增强子和启动子正确插入虫荧光素酶真核表达载体，测序结果与GENEBANK报道序列一致。结论aP2基因增强子和启动子驱动虫荧光素酶表达的真核表达载体构建成功。

Construction and identification of a lucifrase expression vector driven by aP2 enhancer and promoter

Objective To construct a luciferase expression vector driven by aD2 enhancer and promoter, for the further research on aP2 gene expression regulatory mechanism and its signal transduction way. Methods The fragments of aP2 gene promoter and enhancer were obtained by PCR amplification and cloned into the reporter plasmid pGL3-Basic which contained a luciferase gene expression frame. The recombinant eukaryotic expression plasmids in which the expression of the luciferase were drived by the aP2 promoter and enhancer were sequenced. Results The aP2 gene promoter and enhancer with the same sequence as that reported in GENEBANK, were correctly cloned into the eukaryofic reporter vector named pGL3-Basic. Conclusion The tuciferase expression vector driven by aP2 enhancer and promoter was successfully constructed.