| 柚皮素对K562细胞的抑制作用与Caspase酶活性、PCNA蛋白表达的关系 |
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| 引用本文： | 刘明辉,左学兰,胡宁,周颖,冯颖倩. 柚皮素对K562细胞的抑制作用与Caspase酶活性、PCNA蛋白表达的关系[J]. 医学新知杂志, 2008, 18(4): 201-202 |
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| 作者姓名： | 刘明辉 左学兰 胡宁 周颖 冯颖倩 |
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| 作者单位： | 430071,湖北武汉,武汉大学中南医院血液内科 |
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| 摘 要： | 目的本研究旨在探讨柚皮素对K562细胞的生长抑制作用与Caspase酶的活性及细胞增殖核抗原（PCNA）蛋白表达的关系。方法用50μmol／L，200μmol／L的柚皮素分别作用K562细胞后，Caspase分光光度计法检测细胞内Caspase-3和Csapase-9酶活性的改变：用细胞免疫化学的方法检测PCNA蛋白的表达。结果在柚皮素作用下，K562细胞内Caspase-3和Caspase-9活性增强（P〈0．05），PCNA的表达显著降低（P〈0．05）。结论柚皮素对K562细胞的抑制作用可能是通过细胞增值抑制和上调Caspase-9和Csapase-3活性诱导细胞凋亡而实现。
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| 关 键 词： | 柚皮素 CASPASE-3 CASPASE-9 PCNA |
Relation of Apoptosis of K562 Induced by Naringenin to Expression of PCNA and Enzyme Activity Changes of Caspase |
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| Liu Minghui,Zuo Xuelan,Hu Ning,et al. Relation of Apoptosis of K562 Induced by Naringenin to Expression of PCNA and Enzyme Activity Changes of Caspase[J]. Journal of New Medicine, 2008, 18(4): 201-202 |
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| Authors: | Liu Minghui Zuo Xuelan Hu Ning et al |
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| Affiliation: | Liu Minghui,Zuo Xuelan,Hu Ning,et al Department of Hematology,Zhongnan Hospital of Wuhan University,Wuhan 430071 |
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| Abstract: | Objective To investigate the relation of apoptosis of K562 cells induced by naringenin to expression of PCNA and enzyme activity of Caspase. Methods The activity of Caspase-3 and Caspase-9 in K562 cell line induced by naringenin was detected by Caspase Colorimetric Assay. PCNA protein of K562 cell line treated by naringenin was detected by immunhistochemical staining. Results The activity of Caspase-3 and Caspase-9 increased remarkably with treatment of naringenin, while PCNA protein in treatment group decreased remarkably than that in control group. Conclusion The activity of Caspase-3 and Caspase-9 and cell cycle arrest may contribute to the apoptosis of K562 cell in vitro induced by naringenin. |
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| Keywords: | naringenin Caspase-3 Caspase-9 PCNA |
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