| 多巴胺D3受体对胰岛素受体介导的促血管平滑肌细胞增殖作用的影响 |
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| 引用本文： | 黄河飞,韩愈,曾春雨,何多芬,石伟彬,傅春江,杨成明,王旭开,黄岚,周林.多巴胺D3受体对胰岛素受体介导的促血管平滑肌细胞增殖作用的影响[J].高血压杂志,2007,15(12):1008-1011. |
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| 作者姓名： | 黄河飞 韩愈 曾春雨 何多芬 石伟彬 傅春江 杨成明 王旭开 黄岚 周林 |
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| 作者单位： | 第三军医大学大坪医院(野战外科研究所)心内科心血管病研究室 重庆400042(黄河飞，韩愈，曾春雨，何多芬，石伟彬，傅春江，杨成明，王旭开，周林)，第三军医大学新桥医院心内科 重庆400037(黄岚) |
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| 基金项目： | 国家自然基金资助项目(30470728、30672199) |
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| 摘 要： | 目的研究多巴胺D3受体对胰岛素受体介导的促血管平滑肌细胞增殖作用的影响。方法以胚胎大鼠胸主动脉血管平滑肌细胞株(A10)为研究对象,观察在D3受体激动剂作用下,胰岛素受体促血管平滑肌细胞增殖作用的变化。利用3H]-TdR细胞掺入实验观察细胞增殖状况,并利用免疫印迹法观察D3受体对胰岛素受体蛋白表达的影响,初步探讨D3受体影响胰岛素受体介导的促血管平滑肌细胞增殖作用的机制。结果D3受体激动剂(PD128907)本身对血管平滑肌细胞增殖没有影响,但可抑制胰岛素受体介导的促血管平滑肌细胞增殖作用。刺激D3受体可降低胰岛素受体的表达,提示D3受体可能通过影响胰岛素受体的表达,从而影响胰岛素受体促血管平滑肌细胞增殖的过程。结论D3受体对胰岛素受体介导的促血管平滑肌细胞增殖具有一定的抑制作用。
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| 关 键 词： | 多巴胺D_3受体 胰岛素受体 血管平滑肌细胞 高血压 |
Effects of D_3 Dopamine Receptor on Insulin Receptor-mediated Proliferation of Vascular Smooth Muscle Cells |
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| HUANG He-fei,HAN Yu,ZENG Chun-yu,HE Duo-fen,SHI Wei-bin,FU Chun-jiang,YANG Cheng-ming,WANG Xu-kai,HUANG Lan,ZHOU Li..Effects of D_3 Dopamine Receptor on Insulin Receptor-mediated Proliferation of Vascular Smooth Muscle Cells[J].Chinese Journal of Hypertension,2007,15(12):1008-1011. |
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| Authors: | HUANG He-fei HAN Yu ZENG Chun-yu HE Duo-fen SHI Wei-bin FU Chun-jiang YANG Cheng-ming WANG Xu-kai HUANG Lan ZHOU Li |
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| Institution: | HUANG He-fei1,HAN Yu1,ZENG Chun-yu1,HE Duo-fen1,SHI Wei-bin1,FU Chun-jiang1,YANG Cheng-ming1,WANG Xu-kai1,HUANG Lan2,ZHOU Li1.1.Department of Cardiology,Daping Hospital,2.Xinqiao Hospital,The Third Military Medical University,Chongqing City,400042,China |
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| Abstract: | Objective To investigate the effect of D3 dopamine receptor on insulin receptor-mediated proliferation of vascular smooth muscle cells.Methods The A10 cells were used in this study;the effects of D3 receptor on insulin recepuor were determined by immunoblotting.The proliferation of vascular smooth muscle cell was determined by 3H]-TdR incorporation.Results D3 receptors had no effect on vascular smooth muscle cell(VSMC) proliferation,but blocked the insulin-mediated proliferation in A10 cells.Immunoblotting results showed that activation of D3 receptors by PD128907 inhibited the insulin receptor expression,which might involve in the mechanisms of inhibitory effect of D3 receptor on insulin receptor-mediated cell proliferation.Conclusion Activation of D3 receptor inhibits insulin receptor-mediated proliferation of vascular smooth muscle cells. |
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| Keywords: | D_3 dopamine receptor Insulin receptor Vascular smooth muscle cells Hypertension |
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