内皮型一氧化氮合成酶及其mRNA在慢性乙型肝炎肝组织中的表达及意义

目的 研究内皮型一氧化氮合成酶（Endothelial nitric nitric oxide synthase,eNOS）及其mRNA在慢性乙型肝炎（Chronic hepatitisB,CHB）肝组织中的表达及与肝组织病变的关系和临床意义。方法 采用免疫组化及核酸原位杂交等技术对48例CHB肝组织中eNOS的表达进行了观察和分析，同时研究了eNOS对CHB肝组织中转化生长因子－β受体I（Transforming growth factor-β receptorⅠ,TGF-βRⅠ)的表达的影响。结果 eNOS主要表达于肝细胞、窦内皮细胞及血管内皮细胞，以肝细胞为主。连续切片观察，eNOSmRNA的表达与其酶蛋白的表达在阳性细胞数量及分布上基本一致。随CHB肝组织炎症程度和纤维化程度的加重，eNOS表达增强（r＝0．6855，P＜0．55；r＝0．4828，P＜0．05）。随eNOS表达上调，患者PA及血清ALB下降（F=3．1401，P＜0．05；F＝9．1714，P＜0．01）。CHB肝组织内eNOS的表达与TGF－βRⅠ的表达呈明显正相关（r＝0．7781，P＜05）。结论 eNOS可能通过影响TGFβRⅠ在肝组织中的表达，参与CHB肝组织病理损伤过程。

Study on the expression of endothelial nitric oxide synthase and its mRNA in liver tissues of patients with chronic hepatitis B

BACKGROUND: To study the expression of endothelial nitric oxide synthase (eNOS) and its mRNA in liver tissues of patients with chronic hepatitis B (CHB) and the relationship between its expression and pathological changes of hepatic tissues and the clinical significance. METHODS: 48 cases of specimens of liver tissues with CHB were observed and analyzed using immunohistochemical method and in situ nucleic acid hybridization. RESULTS: eNOS was expressed in hepatocytes, sinusiod endothelial cells and vessel endothelial cells, being predominantly expressed in hepatocytes. The number and distribution of eNOS mRNA positive cells were similiar to those of its protein. Increase in expression of eNOS was correlated with degrees of inflammatory activity and fibrosis (r=0.6855 [P<0.05]; r=0.4828 [P<0.05]; respectively), and with decreasing PA and ALB (F=3.4101 [P<0.05]; F=9.1714 [P<0.0]). Changes of expression of TGF-alpha-R were parallel to those of eNOS. CONCLUSIONS: eNOS may be involved in pathological lesion of CHB by affecting the expression of TGF-R in liver tissues with CHB.