细胞-支架构建方式的牙周组织工程实验研究

目的　观察评价细胞 支架构建方式的组织工程方法对牙周组织再生修复的影响和意义。方法　体外培养动物自体牙周膜细胞 (PDLCs) ,传代扩增后接种到纳米羟基磷灰石材料 (nHAC)三维支架上 ,扫描电镜观察PDLCs在nHAC支架上的附着及生长情况 ;同时 ,将PDLCs nHAC复合物植入动物牙周组织缺损中 ,术后 8周观察 ,评价其牙周组织的再生情况。结果　扫描电镜显示 ,nHAC具有良好的多孔网状结构 ,PDLCs在nHAC上贴附牢固 ,生长旺盛。动物实验可见 ,PDLCs nHAC植入组较对照组有更多的新生牙槽骨、新生牙周膜和新生牙骨质生成 ,缺损处牙周组织几乎完全再生 ,且未见上皮长入。结论　应用细胞 支架构建方式的牙周组织工程方法能获得理想的牙周组织再生和重建。

A study on the effects of cells and scaffolds tissue engineering on the periodontal regeneration

Objective To observe the effects of cells and scaffolds tissue engineering on the periodontal regeneration,and to evaluate the feasibility of nano-Hap-collagen (nHAC) as the scaffold material for periodontal tissue engineering.Methods Dog autogenous periodontal ligament cells (PDLCs) cultured in vitro were collected and seeded on the three-dimensional framework of nHAC. The cell growth in the scaffolds was observed by scanning electron microscope. And then the PDLCs-nHAC composites were transplanted into man-made periodontal defects,and the groups filled with nothing or filled only with nHAC were the controls. The dogs were sacrificed after 8 weeks and the periodontal regeneration was observed histologically.Results Scanning electron microscope showed the porous structure of nHAC and the eugonic growth of cells in the nHAC scaffolds. The histological observation showed that the PDLCs-nHAC groups exhibited more new bone,new periodontal ligament and new cementum occupying the majority of the defects than the control groups,and the epitheliums were not observed.Conclusions Periodontal regeneration could be enhanced by the cells and scaffolds tissue engineering,and the PDLCs and nHAC could be used as the seed cell and the scaffold material for periodontal tissue engineering.