| 贵州豆豉激酶组分的Tricine-SDS-PAGE电泳方法研究 |
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| 引用本文： | 高秀丽,宋智魁,王清忱,孟小夏,舒阳,张敏,王鹏娇.贵州豆豉激酶组分的Tricine-SDS-PAGE电泳方法研究[J].贵阳医学院学报,2010,35(6):575-577. |
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| 作者姓名： | 高秀丽 宋智魁 王清忱 孟小夏 舒阳 张敏 王鹏娇 |
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| 作者单位： | 贵阳医学院药学院;贵州省人民医院; |
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| 摘 要： | 目的：建立一种能够稳定检测贵州豆豉激酶组分的Tricine-SDS-PAGE电泳方法。方法：采用Tricine-SDS-PAGE电泳方法,分离胶浓度为12%,交联度为3%,以blue-silver染色法进行染色,对贵州豆豉激酶组分进行分析。结果：贵州豆豉激酶组分经Tricine-SDS-PAGE电泳后,在蛋白Marker在20.1～43 kDa之间,出现5条较清晰的条带。结论：Tricine-SDS-PAGE电泳方法稳定可靠,能够用于贵州豆豉激酶组分的检测。
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| 关 键 词： | 电泳 贵州豆豉激酶组分 纤溶酶 发酵 |
A Tricine-SDS-PAGE Electrophoresis Method for Determination of Kinase Components in Guizhou Douchi |
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| GAO Xiuli,SONG Zhikui,WANG Qingchen,MENG Xiaoxia,SHU Yang,ZHANG Min,WANG Pengjiao.A Tricine-SDS-PAGE Electrophoresis Method for Determination of Kinase Components in Guizhou Douchi[J].Journal of Guiyang Medical College,2010,35(6):575-577. |
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| Authors: | GAO Xiuli SONG Zhikui WANG Qingchen MENG Xiaoxia SHU Yang ZHANG Min WANG Pengjiao |
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| Institution: | 1.School of Pharmacology,Guiyang Medical College,Guiyang 550004,Guizhou,China;2.Guizhou Provincial Pelple′s Hospital,Guiyang 550002,Guizhou,China) |
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| Abstract: | Objective: To establish a tricine-SDS-PAGE method for stable determination of kinase components in Guizhou douchi.Method: Tricine-SDS-PAGE was used,of which the gel concentration and cross-linking density were 12% and 3%,respectively.After electrophoresis,the gel was stained with blue-silver method.Results: Five bands between 20.14 kd and 43 kd were found on the electrophoretogram.Conclusion: Tricine-SDS-PAGE could detect the components of Guizhou douchi steadily and reliably. |
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| Keywords: | electroproresis kinase components in Guizhou douchi plasmin fermentation |
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