| Early apoptosis and cell death induced by ATX-S10Na （ Ⅱ ）-mediated photodynamic therapy are Bax- and p53-dependent in human colon cancer cells |
| |
| 作者姓名： | Mitsunaga M Tsubota A Nariai K Namiki Y Sumi M Yoshikawa T Fujise K |
| |
| 作者单位： | Makoto Mitsunaga（Institute of Clinical Medicine and Research, Jikei University School of Medicine, Kashiwa, Chiba, Japan;Division of Gastroenterology and Hepatology, Department of Internal Medicine,Jikei University School of Medicine, Tokyo, Japan） ;
Akihito Tsubota（Institute of Clinical Medicine and Research, Jikei University School of Medicine, Kashiwa, Chiba, Japan） ;
Kohichi Nariai（Institute of Clinical Medicine and Research, Jikei University School of Medicine, Kashiwa, Chiba, Japan） ;
Yoshihisa Namiki（Institute of Clinical Medicine and Research, Jikei University School of Medicine, Kashiwa, Chiba, Japan） ;
Makoto Sumi（Institute of Clinical Medicine and Research, Jikei University School of Medicine, Kashiwa, Chiba, Japan） ;
Tetsuya Yoshikawa（Institute of Clinical Medicine and Research, Jikei University School of Medicine, Kashiwa, Chiba, Japan） ;
Kiyotaka Fujise（Institute of Clinical Medicine and Research, Jikei University School of Medicine, Kashiwa, Chiba, Japan;Division of Gastroenterology and Hepatology, Department of Internal Medicine,Jikei University School of Medicine, Tokyo, Japan） ; |
| |
| 基金项目： | Supported by a grant from the Jikei University School of Medicine |
| |
| 摘 要： | AIM： To investigate the roles of Bax and p53 proteins in photosensitivity of human colon cancer cells by using lysosome-localizing photosensitizer, ATX-S10Na （Ⅱ）. METHODS： HCT116 human colon cancer cells and Bax-null or p53-null isogenic derivatives were irradiated with a diode laser. Early apoptosis and cell death in response to photodynamic therapy were determined by MTT assays, annexin Ⅴ assays, transmission electron microscopy assays, caspase assays and western blotting. RESULTS： Induction of early apoptosis and cell death was Bax- and p53-dependent. Bax and p53 were required for caspase-dependent apoptosis. The levels of anti-apoptotic Bcl-2 family proteins, Bcl-2 and Bcl-xL, were decreased in Bax- and p53-independent manner. CONCLUSION： Our results indicate that eady apoptosis and cell death of human colon cancer cells induced by photodynamic therapy with lysosome-localizing photosensitizer ATX-S10Na （Ⅱ） are mediated by p53- Bax network and low levels of Bcl-2 and Bcl-xL proteins. Our results might help in formulating new therapeutic approaches in photodynamic therapy.
|
| 关 键 词： | 结肠癌 癌细胞 细胞凋亡 细胞死亡 光动力疗法 ATX-S10Na 介导 BAX P53 |
| 收稿时间： | 2006 Oct 20 |
Early apoptosis and cell death induced by ATX-S10Na (II)-mediated photodynamic therapy are Bax- and p53-dependent in human colon cancer cells |
| |
| Mitsunaga M,Tsubota A,Nariai K,Namiki Y,Sumi M,Yoshikawa T,Fujise K.Early apoptosis and cell death induced by ATX-S10Na (II)-mediated photodynamic therapy are Bax- and p53-dependent in human colon cancer cells[J].World Journal of Gastroenterology,2007,13(5):692-698. |
| |
| Authors: | Mitsunaga Makoto Tsubota Akihito Nariai Kohichi Namiki Yoshihisa Sumi Makoto Yoshikawa Tetsuya Fujise Kiyotaka |
| |
| Institution: | Institute of Clinical Medicine and Research, Jikei University School of Medicine, 163-1 Kashiwa-shita, Kashiwa, Chiba 277-8567, Japan. |
| |
| Abstract: | AIM: To investigate the roles of Bax and p53 proteins in photosensitivity of human colon cancer cells by using lysosome-localizing photosensitizer, ATX-S10Na (II). METHODS: HCT116 human colon cancer cells and Bax-null or p53-null isogenic derivatives were irradiated with a diode laser. Early apoptosis and cell death in response to photodynamic therapy were determined by MTT assays, annexin V assays, transmission electron microscopy assays, caspase assays and western blotting. RESULTS: Induction of early apoptosis and cell death was Bax- and p53-dependent. Bax and p53 were required for caspase-dependent apoptosis. The levels of anti-apoptotic Bcl-2 family proteins, Bcl-2 and Bcl-x(L), were decreased in Bax- and p53-independent manner. CONCLUSION: Our results indicate that early apoptosis and cell death of human colon cancer cells induced by photodynamic therapy with lysosome-localizing photosensitizer ATX-S10Na (II) are mediated by p53-Bax network and low levels of Bcl-2 and Bcl-x(L) proteins. Our results might help in formulating new therapeutic approaches in photodynamic therapy. |
| |
| Keywords: | Photodynamic therapy ATX-S10Na （Ⅱ） Apoptosis Bax p53 |
| 本文献已被 维普 PubMed 等数据库收录！ |
|
