大鼠烧伤后血浆应激激素和脾细胞免疫功能变化的动态观察

本实验采用大鼠烧伤应激模型,同时观测伤后血浆应激激素及脾细胞免疫功能的变化。发现伤后24h内脾细胞SBT明显降低时,体外ConA刺激的MSBT却正常或稍增高,呈现伤后脾细胞免疫活性的第1次分离。此现象可能系同时发生显著变化的皮质酮和儿茶酚胺引起。伤后72h的第2次分离则是SBT显著升高和MSBT显著降低,而此时应激激素已趋正常,故可能是其它因素所致。此外,脾细胞产生IL2在伤后一直处于低水平,早期可能与皮质酮有关。本实验提示应激激素至少参与了伤后早期的免疫调节,而烧伤免疫抑制是多因素综合作用的结果。

Postburn Kinetic Changes of Stress Hormones and Splenocyte Immune Functions in Rats

After the major burn a multitude of immunologic alterations develop. Although the roles of suppressor cells and serum inhibitory factors in the mechanism of postburn immunosuppression are well known, the role of the neuroendo-crine response is still not understood. In this paper determining stress hormones and splenocyte immune functions are simultaneously focused on in deeply burned rats with 25% BSA. The changes of plasma corticosterone (B) and catecholamine are dominant within 24 h postburn(PB), and soon return to normal. The endogenous activity of splenocytes (SBT) decreases at 12 h PB. while the ability of spleno-cytes responsing to ConA in vitro (MSBT) is almost unchanged. The pathological lesions of spleen may be affected by increasing levels of plasma B. When most of the stress hormones return to normal at 72 h PB the SBT and MSBT become different. This may be influenced by many other factors rather than the neuroendocrine system. The differences between SBT and MSBT suggest thai SBT may reflect the more real condition of lymphocytes in vivo than MSBT in vitro. Following burns the roles of streess hormones actually participate in the immune regulation such as plasma B and catecholamine act in the early postburn stage.