| 高效液相色谱法测定大豆胚轴中大豆皂甙Ba和Bb含量 |
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| 引用本文： | 全吉淑,尹学哲,工藤重光. 高效液相色谱法测定大豆胚轴中大豆皂甙Ba和Bb含量[J]. 延边医学院学报, 2002, 25(4): 235-237 |
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| 作者姓名： | 全吉淑 尹学哲 工藤重光 |
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| 作者单位： | 延边大学医学院生物化学教研室，延边大学医学院附属医院呼吸内科，东北食效科学研究所 吉林，延吉133000，吉林，延吉133000，日本，青森县，青森市0300842 |
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| 摘 要： | [目的 ]建立大豆皂甙Ba和Bb的含量测定方法 .[方法 ]应用反相高效液相色谱法 ,采用ODS AM 30 3色谱柱 (YMC ,4 6mm× 2 5 0mm ,5 μm) ,以含 1mL/L三氟乙酸的乙腈 水 ( 40∶6 0 )为流动相 ,检测波长为 2 10nm .[结果 ]大豆皂甙Ba和Bb分别在 2 2 4～ 11 2 0 μg和 2 35～ 11 80 μg范围内线性关系良好 ,日内RSD分别为 3 15 %和 3 98% .[结论 ]本方法准确度高 ,重现性好 ,适用于B类大豆皂甙的含量测定 .
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| 关 键 词： | 皂苷类 植物,药用 色谱法,高压液相 |
| 文章编号： | 1000-1824(2002)04-0235-02 |
| 修稿时间： | 2002-09-17 |
Determination of soyasaponin Ba and Bb in Soybean Hypocotyl by high performance liquid chromatography |
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| QUAN Ji-shu,YIN Xue-zhe,KUDOU Shigemitsu. Determination of soyasaponin Ba and Bb in Soybean Hypocotyl by high performance liquid chromatography[J]. Journal of Medical Science Yanbian University, 2002, 25(4): 235-237 |
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| Authors: | QUAN Ji-shu YIN Xue-zhe KUDOU Shigemitsu |
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| Affiliation: | QUAN Ji-shu1,YIN Xue-zhe2,KUDOU Shigemitsu3 |
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| Abstract: | OBJECTIVETo establish a method for determination of soyasaponin Ba and Bb.METHODSSoyasaponin Ba and Bb were detected by a reversed-phase liquid chromatographic method. The separation was performed in ODS-AM-303 column (YMC, 4.6mm×250mm, 5μm), with acetonitrile-water (40∶60) containing 1mL/L trifluoroacetic acid as a mobile phase. The detect wavelength was set at 210nm.RESULTSThe method was proved to be linear in the range of 2 24～11 20μg and 2 35～11 80μg, and the intra-day coefficients of variation of assay for soyasaponin Ba and Bb were 3 15% and 3 98%, respectively. CONCLUSIONThe method is accurate and reproducible, suitable for determination of soyasaponin Ba and Bb. |
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| Keywords: | saponins plants medicinal chromatography high pressure liquid |
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