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| 去甲斑蝥素诱导人肝癌细胞凋亡的研究 | |
| 引用本文： | 李先茜,邵世和,傅桂莲,韩晓红,高虹.去甲斑蝥素诱导人肝癌细胞凋亡的研究[J].山东医药,2010,50(25):9-11. |
| 作者姓名： | 李先茜 邵世和 傅桂莲 韩晓红 高虹 |
| 作者单位： | 1. 江苏大学基础医学与医学技术学院,江苏镇江212013;上海中医药大学附属普陀医院 2. 江苏大学基础医学与医学技术学院,江苏镇江,212013 3. 迈阿密大学医学院 4. 上海中医药大学附属普陀医院 |
| 基金项目： | 上海中医药大学附属普陀医院科研基金 |
| 摘 要： | 目的探讨去甲斑蝥素（NCTD）对人肝癌细胞SMMC-7721凋亡的影响。方法用MTT方法检测人肝癌细胞SMMC-7721的生长抑制率。应用流式细胞仪检测细胞周期和凋亡。结果 NCTD对人肝癌细胞SMMC-7721有生长抑制作用,随浓度升高、时间延长作用增强,呈剂量和时间效应关系。流式细胞仪示SMMC-7721细胞的S期细胞明显增多,G0/G1期细胞减少,凋亡率上升（P〈0.05或〈0.01）。结论 NCTD能显著抑制人肝癌SMMC-7721细胞的生长,其可能与抑制SMMC-7721细胞增殖,诱导细胞凋亡有关。 |
| 关 键 词： | 去甲斑蝥素 肝肿瘤 细胞凋亡 |
Effects of norcantharidin induced apoptosis with human hepatoma carcinoma |
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| LI Xian-qian,SHAO Shi-he,FU Gui-lian,HAN Xiao-hong,GAO Hong.Effects of norcantharidin induced apoptosis with human hepatoma carcinoma[J].Shandong Medical Journal,2010,50(25):9-11. | |
| Authors: | LI Xian-qian SHAO Shi-he FU Gui-lian HAN Xiao-hong GAO Hong |
| Institution: | 1 School of Medical Science and Laboratory Medicine,Jiangsu University,Zhenjiang 212013,P.R.China) |
| Abstract: | Objective To investigate the effects of norcantharidin（NCTD）induced apoptosis in SMMC-7721 cell lines with human hepatoma carcinoma.Methods The rate of SMMC-7721 cell growth inhibition was measured by MTT method.Flow cytometry was used to measure the induction of cell cycle arrest and apoptosis.Results Norcantharidin inhibited SMMC-7721 cell growth in a time-and dose-dependent manner.The flow cytometric profiles revealed that NCTD led to the shift from 39.83% up to 56.82% of the S phase and from 70.03% down to 43.18% of the G0/G1 phase cells in percentage of SMMC-7721 cells,with the increased rate of cell apoptosis.Conclusions NCTD could inhibit the growth of SMMC-7721 cell lines with human hepatoma carcinoma.The mechanism might correlate with inhibition of cell proliferation,induction of cell apoptosis. |
| Keywords: | norcantharidin liver neoplasms apoptosis |
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