| Cyclins/DNA多参数流式细胞术分析细胞增殖 |
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| 引用本文： | 龚建平,韦伟,舒丹,陶德定. Cyclins/DNA多参数流式细胞术分析细胞增殖[J]. 华中科技大学学报(医学版), 1999, 28(3): 6 |
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| 作者姓名： | 龚建平 韦伟 舒丹 陶德定 |
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| 作者单位： | 同济医科大学附属同济医院分子医学中心,武汉,430030 |
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| 基金项目： | 国家自然科学基金,卫生部基金 |
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| 摘 要： | 应用Ｃｙｃｌｉｎｓ／ＤＮＡ双参数流式细胞术，分析有丝分裂原刺激后的正常人外周血Ｔ淋巴细胞的细胞增殖（特别是Ｇ１期细胞增殖），同时与Ｋｉ－６７／ＤＮＡ双参数法进行比较。结果表明，Ｃｙｃｌｉｎｓ／ＤＮＡ法比Ｋｉ－６７／ＤＮＡ法早２０ｈ发现增殖的淋巴细胞，有丝分裂原刺激后４８ｈ，Ｃｙｃｌｉｎｓ／ＤＮＡ法又比Ｋｉ－６７／ＤＮＡ法多检出２０％的增殖淋巴细胞。结论：Ｃｙｃｌｉｎｓ／ＤＮＡ双参数流式细胞术分析细胞增殖具可行性、实用性、科学性和全面性。
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| 关 键 词： | 细胞增殖；流式细胞术；细胞周期素 |
Cell Proliferation Analysis by Cyclins/DNA Multiparameter Flow Cytometry |
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| Gong Jianping,Wei Wei,Shu Danet al Molecular Medicine Center,Tongji Hospital,Tongji Medical University,Wuhan. Cell Proliferation Analysis by Cyclins/DNA Multiparameter Flow Cytometry[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 1999, 28(3): 6 |
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| Authors: | Gong Jianping Wei Wei Shu Danet al Molecular Medicine Center Tongji Hospital Tongji Medical University Wuhan |
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| Affiliation: | Gong Jianping,Wei Wei,Shu Danet al Molecular Medicine Center,Tongji Hospital,Tongji Medical University,Wuhan 430030 |
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| Abstract: | The mitogen stimulated normal human T lymphocytes proliferation (especially G1 phase proliferation) was analyzed by using a developed cyclins/DNA multiparameter flow cytometry and Ki67/DNA multiparameter method. The results showed that the developed method detected proliferated T lymphocytes 20 earlier than Ki67 method, and 20 % more proliferated cells than Ki67 method at 48 h after mitogen stimulation. It was concluded that cyclins/DNA multiparameter folw cytometry is more practicable, reasonable and easy for cell proliferation analysis in contrast to the previous methods, especially for cell proliferation in early G1 |
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| Keywords: | cell proliferation flow cytometrycy Cycling |
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