ADRB2基因多态性与aSAH迟发性脑血管痉挛的关联性研究

目的:探讨中国北方汉族人群中,动脉瘤性蛛网膜下腔出血(aSAH)后迟发性脑血管痉挛(DCVS)与β2肾上腺素能受体(ADRB2)基因多态性的关系。方法:aSAH患者206例纳入研究,按照是否合并DCVS分为DCVS组128例和无DCVS组78例,采用聚合酶链反应-限制性片段长度多态性法检测ADRB2基因A46G位点和C79G位点多态性。结果:单因素分析结果显示ADRB2基因A46G位点等位基因模型(A vs.G)和显性基因模型(A/A vs.A/G+G/G)均与DCVS发生相关;多因素Logistics回归分析结果显示ADRB2基因+46位点等位基因G和基因型(A/G+G/G)与aSAH患者发生DCVS的危险因素(OR=1.414,95%CI:1.142~4.817,P=0.039;OR=1.337,95%CI:1.076~3.191,P=0.045);C79G位点各基因模型与DCVS相关性均无统计学意义。结论:对于中国北方汉族aSAH患者,ADRB2基因A46G位点多态性与DCVS发生相关。

Association between Polymorphism of Beta-2 Adrenergic Receptor and Delayed Cerebral Vasospasm in Patients with Aneurysmal Subarachnoid Hemorrhage

To investigate the genetic association between Beta-2 Adrenergic Receptor (ADRB2)gene polymorphism and delayed cerebral vasospasm (DCVS) in patients with aneurysmal subarachnoid hemorrhage (aSAH) in the Han descent population in North of China. Methods: Two hundred and six aSAH patientswere recruited. According to whether DCVS occurred, cases were divided into the DCVS group (n=128) and thenon-DCVS group (n=78). Polymerase chain reaction-restriction fragment length polymorphism combined withDNA direct sequencing technique were used to determine the polymorphism of the ADRB2 gene. Results: Univariate analysis showed independent association between allele model (A vs. G) and the dominant model (A/Avs. A/G + G/G) of ADRB2 gene A46G polymorphism and DCVS. Multivariate Logistic regression analysisshowed that ADRB2 gene +46 position allele G and genotype (A/G + G/G) were risk factors of DCVS for aSAHpatients (OR=1.414, 95%CI: 1.142~4.817, P=0.039; OR=1.337, 95%CI: 1.076~3.191, P=0.045). However, therewas no significant difference in the frequencies of genotype and allele of C79G polymorphism between theDCVS group and the non-DCVS group. Conclusion: For aSAH patients in the Han descent population in theNorth of China, A46G polymorphism ofADRB2 gene may closely related to DCVS.