帕金森病患者血浆中亲环素A的含量及其与炎症因子的相关性分析

目的探讨帕金森病患者血浆中亲环素A(Cy PA)的含量变化并分析其与炎症因子的相关性。方法收集2015年6月至2017年6月我院神经内科收治的帕金森病患者80例(帕金森组),另选取同期健康体检者80例为对照(对照组)。全自动生化分析仪检测Cy PA、超敏C反应蛋白(hs-CRP)、肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)和白细胞介素1β(IL-1β)含量;分析Cy PA含量与hs-CRP、TNF-α、IL-6和IL-1β含量的相关性;实时荧光定量PCR(RT-q PCR)和免疫印迹法(Western Blot)检测LPS处理后Cy PA mRNA和蛋白表达变化;小干扰RNA(siRNA)敲减Cy PA的表达并验证后,采用酶联免疫分析法(ELISA)检测hs-CRP、TNF-α、IL-6和IL-1β的含量。结果帕金森组患者血浆Cy PA含量及血清中炎症因子含量高于对照组(P0.05);Cy PA含量与TNF-α、IL-6和IL-1β呈正相关关系(P0.05),与hs-CRP无相关性(P0.05)。LPS处理BV-2小胶质细胞可使Cy PA mRNA和蛋白质含量表达增加(P0.05)。siRNA敲减Cy PA的表达可降低TNF-α、IL-6和IL-1β的含量(P0.05),对hs-CRP含量则无影响(P0.05)。结论帕金森病患者血浆Cy PA含量升高可促进小胶质细胞炎症因子分泌,Cy PA可作为抑制或缓解小胶质细胞炎症状态的靶点。

Plasma level of cyclophilin A and its correlation with inflammatory factors in patients with Parkinson's disease

Objective To investigate the changes in the plasma level of cyclophilin A (CyPA) in patients with Parkinson's disease (PD) and its correlation with inflammatory factors.Methods Eighty patients with PD who were admitted to our hospital from June 2015 to June 2017 were assigned to PD group. Eighty healthy volunteers within the same period were assigned to control group. An automatic biochemical analyzer was used to determine the levels of CyPA, hypersensitive C-reactive protein (hs-CRP), tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β). The correlation of the CyPA level with the levels of hs-CRP, TNF-α, IL-6, and IL-1β was analyzed. RT-PCR and Western blot were used to determine the changes in the mRNA and protein expression of CyPA after lipopolysaccharide (LPS) treatment. The levels of hs-CRP, TNF-α, IL-6, and IL-1β were measured by enzyme-linked immunosorbent assay after knockdown of CyPA expression using small interfering RNA (siRNA).Results The plasma level of CyPA and serum levels of inflammatory factors were significantly higher in the PD group than in the control group (P<0.05). The CyPA level was positively correlated with the levels of TNF-α, IL-6, and IL-1β (P<0.05). There was no correlation between the CyPA level and the hs-CRP level (P>0.05). LPS treatment of BV-2 microglial cells significantly increased the mRNA and protein expression of CyPA (P<0.05). The knockdown of CyPA by siRNA significantly reduced the levels of TNF-α, IL-6, and IL-1β (P<0.05) but had no effect on the hs-CRP level (P>0.05).Conclusions An increased plasma level of CyPA promotes the secretion of inflammatory factors by microglial cells in patients with PD. CyPA can be used as a target to inhibit or alleviate the inflammatory state of microglial cells.