溃疡性结肠炎相关性结肠癌差异表达的miRNA的筛选研究

[目的]分析结肠癌与正常结肠黏膜、结肠炎肠黏膜的微小RNA（miRNA）表达谱差异.[方法]通过建立溃疡性结肠炎（ulcerative colitis,UC）及UC相关性结肠癌（ulcerative colitis related colorectal cancer,UCRCC）小鼠模型,抽提、纯化RNA,加入荧光标记后与miRNA寡核甘酸基因芯片（Affymetrix公司）杂交,应用SAM软件进行数据分析,对有显著差异的miRNA进行实时荧光定量PCR验证,采用靶基因分析软件分析miRNA功能.[结果]has-miR-194、has-miR-215、has-miR-93、has-miR-192、has-miR-92a、has-miR-29b、has-miR-20a等7个miRNA在肿瘤组织中显著上调[中位假基因验出率（FDR）＜5％],has-miR-1231、has-miR-195、has-miR-143、has-miR-145等4个miRNA显著下调（FDR＜5％）.[结论]UCRCC与正常结肠黏膜、UC肠黏膜之间存在明显的miRNA差异表达,这些miRNA的差异性表达可能与UCRCC的发病有关.

Screening of differentially expressed miRNA in ulcerative colitis related colorectal cancer

[Objective]To investigate the differential expression of miRNA in colon between ulcerative colitis （UC） and ulcerative colitis related color＇ectal cancer （UCRCC）. [Methods]UC mouse model was built by dextran sodium sulfate, and UCRCC mouse model by dextran sodium sulfate. RNAs were extrac- ted from the colon, purified and hybridized with fluorescence-labeled miRNA oligouncleotide gene chip. Real-time fluorescence quantitative PCR was used to verify the expression variation of miRNA. SAM was employed for the data analysis. [Results] The up-regulated miRNAs in colon cancer included has-miR-194, has-miR-215, has-miR-93, has-miR-192, has-miR-92a, has-miR-92b, and has-miR-20a （median false dis- covery rate 〈5%）, while the down-regulated miRNAs were has-miR-1231, has-miR-195, has-miR-143, has-miR-194, and has-miR-145 （median false discovery rate 〈5%） [Conclusion] Significant differential ex- pression of miRNA was found between UC mouse and UCRCC mouse, which may be related to the onset of colorectal cancer.