32P-玻璃微球间质给药体内生物学分布与代谢

目的 观察32P-玻璃微球(32P-GMS)间质给药后体内生物学分布与代谢.方法 120只小鼠分别经肝脏或肌肉注射32P-GMS,每只(1.80±0.05)MBq/50μl,不同时间点处死取血及脏器计算每克组织百分注射剂量率(%ID/g);12只大鼠肝脏、肌肉每只注射(18.0±0.5)MBq/0.5 ml,收集排泄物测累积排泄率.结果 小鼠肝脏组给药肝叶%ID/g 0时最高为1.38,15 nin降至0.71,非给药肝叶峰值15 min为0.52,4 h之后左右肝叶无区别;肺组织计数率值1 h内上升,肝肺累积分流率为37.9%.肌肉组注射点%ID/g0时为31.47,15 nin～8 h为25.06～11.92(中值20.97),12 h为8.70,24 h～14 d为3.54～2.02(中值2.51),其他主要脏器放射性接近本底水平.大鼠肝脏组粪便和尿液14 d累积排泄率分别为0.0751%和0.0586%;肌肉组分别为0.0401%和0.0385%.结论 32P-GMS间质注射适用于除肝脏以外的体内组织脏器恶性实体瘤的治疗.

Biodistribution and metabolism of phosphorus-32 glass microspheres through Interstitial administration

Objective To observe the biodistribution and the metabolism of phospborus-32 glass microspheres (32P-GMS) through interstitial administration. Methods Thirty-two P-GMS were injected into liver and muscle of 120 mice respectively by means of interstitial administration with dose of (1.80±0.05) MBq/50 μl. All of the mice were killed at different time points, blood was taken out and major or-gans were dissected,and the injection dosage rate (% ID/g) was measured. The stool and urine were col-lected after interstitial injection both in liver and muscle of 12 rata with the dose of (18.0±0.5) MBq/0.5 ml,then the accumulative radioactivity counts percentage was measured. Results In liver injection group ,the highest point of % ID/g was 1.38 at start time after administration ,and decreased to 0.71 at 15 rain. The % ID/g value at 15 min was 0.52 in none injected liver lobes,and there was the similar tendency between left and right liver lobes after 4 h. The radioactivity in lung was increased obviously in 1 h,and the liver-lung accumulate split stream rate was 37.9%. In muscle injection greup,the highest % ID/g at start time point was 31.47 after administration,25.06 to 11.92 in 15 rain to 8 h (midvalue was 20.97) ,8.70 at 12 h,and 3.54 to 2.02 in 24 h to 14 days (midvalue was 2.51). The radioactivity of other organs and tissues was close to the background level. The accumulative radioactivity counts percentage of stool and u-rine was 0.0751% and 0.0586% respectively in rat liver group, and 0.0401% and 0.0385 % respectively in rat muscle group. Conclusion 32p-GMS interstitial administration may have great potential to treat the malignancy solid tumor in all organs and tissues except liver.