G—CSF对颈动脉狭窄模型兔内皮祖细胞的动员作用

目的观察粒细胞集落刺激因子（Granuloeyte Colony Stimulating Factor，G—CSF）对动脉狭窄模型兔外周血内皮祖细胞（Endothelial Progenitor Cells．EPCs）数量的影响。方法以颈动脉狭窄模型兔为研究对象，随机分为两组，G—CSF注射组（n=14）和对照组（n=10），两组动物分别予以25μg/kg·d的G—CSF和等量的生理盐水腹部皮下注射，连续注射7d后，用密度梯度离心法从外周血获取单个核细胞。将其接种在人纤维连接蛋白包被培养板，培养7天后贴壁细胞进行化学分析。激光共聚焦显微镜鉴定Dil—acLDL和HTC—UEA-1双染色阳性细胞为正在分化的EPCs。用倒置荧光显微镜进行细胞计数。结果G—CSF组外周血内皮祖细胞比对照组明显增多，两组差异有统计学意义（t=4．815P〈0．001）。结论短期应用G—CSF对颈动脉狭窄模型兔外周血内皮祖细胞有动员作用。

Mobilization effect of G-CSF on the number of circulating endothelial progenitor cells in rabbits of carotid artery stenosis animal model

Objective To observe the effect of the number and function of endothelial progenitor cells in rabbits of carotid artery stenosis animal model. Methods Twenty-four rabbits of carotid artery stenosis animal model were enrolled, those rabbits were randomly divided into G-CSF injection group（n=14） and control group（n=10）. Rabbits were injected daily with 25μg/kg recombinant human G-CSF or 0.9% NaCl SC for 7 consecutive days respectively. On the eighth day of treatment, mononuclear cells were isolated from peripheral blood in 24 rabbits by Histopaque density gradient centrifugation, and then the cells were plated on fibronectin-coatod culture dishes. At day 7 of culture, the immunohistochemical characterization of the adherent cells was performed. EPCs were characterized as adherent cells which were double positive for Dil-acLDL- uptake and FITC-UEA-1 binding by direct fluorescent staining demonstrated under a laser scanning confocal microscope and evaluated the number of EPCs with an inverted fluorescent microscope. Results The number of EPCs was significantly greater in PB-MNCs from the G-CSF-treated group than from those treated with 0.9% NaCl SC （t=4.815 P 〈0.001）. Conclusions G-CSF increases endothelial progenitor cell mobilization and enhances their migration and proliferative function.