Fabrication of protein chips based on 3-aminopropyltriethoxysilane as a monolayer |
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Authors: | Ling-Sheng Jang Hao-Juin Liu |
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Institution: | (1) Department of Electrical Engineering and Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan, Taiwan;(2) 1 University Road, Tainan, 701, Taiwan |
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Abstract: | Although 3-aminopropyltriethoxysilane (APTES) is widely adopted as a monolayer in biosensors, experimental silanization takes
at least 1 h at high temperature. Therefore, the feasibility of the silanization with APTES in a short reaction time and at
room temperature was investigated. The surface modification of glass slides using a self-assembled monolayer of APTES with
a concentration of 10% was studied by immobilizing FITC. APTES was successfully immobilized on the glass slide. The effect
of reaction temperature and time of silanization were investigated. Various silanization conditions of APTES were examined
by contact angle measurement and fluorescence microscopy. The surface of glass patterns with a gold thin film as background
was characterized by determining the fluorescent intensities following the immobilization of fluorescein isothiocyanate (FITC),
protein A-FITC, antimouse IgG-FITC and sheep anti-bovine albumin-FITC. The normalized fluorescent intensity indicated that
a short period (4 min) of silanization at 25°C suffices to form an APTES thin film by the immobilization of protein A on a
glass surface. Such a condition does not require microheaters and temperature sensors in a microfluidic system, which will
significantly reduce the manufacturing process, cost, and reaction time in the future. |
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Keywords: | Self-assembled monolayer APTES Protein chip |
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