In vivo fluorescence lifetime optical projection tomography |
| |
| Authors: | McGinty James Taylor Harriet B Chen Lingling Bugeon Laurence Lamb Jonathan R Dallman Margaret J French Paul M W |
| |
| Affiliation: | 1Photonics Group, Department of Physics, Imperial College London, SW7 2AZ, UK;2Division of Cell and Molecular Biology, Department of Life Sciences, Imperial College London, SW7 2AZ, UK;3Centre for Integrative Systems Biology, Department of Life Sciences, Imperial College London, SW7 2AZ, UK |
| |
| Abstract: | We demonstrate the application of fluorescence lifetime optical projection tomography (FLIM-OPT) to in vivo imaging of lysC:GFP transgenic zebrafish embryos (Danio rerio). This method has been applied to unambiguously distinguish between the fluorescent protein (GFP) signal in myeloid cells from background autofluorescence based on the fluorescence lifetime. The combination of FLIM, an inherently ratiometric method, in conjunction with OPT results in a quantitative 3-D tomographic technique that could be used as a robust method for in vivo biological and pharmaceutical research, for example as a readout of Förster resonance energy transfer based interactions.OCIS codes: (170.3650) Lifetime-based sensing, (170.6900) Three-dimensional microscopy, (170.6920) Time-resolved imaging, (170.6960) Tomography |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
