bFGF对局灶性脑缺血大鼠脑组织GRP78表达的影响

目的研究大鼠脑缺血再灌注后对葡萄糖调节蛋白78(glucose-regulated protein78,GRP78)表达的影响,探讨碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)对脑组织缺血再灌注神经元GRP78的调节作用及机制。方法应用线栓法制作大鼠局灶性脑缺血再灌注模型,大脑中动脉阻塞2h再灌注损伤12h,采用TUNEL法、免疫组化检测海马及皮质内神经元凋亡和GRP78的表达。结果 sham组,海马及皮质偶见凋亡细胞,皮质及海马神经元内少见GRP78免疫反应阳性细胞;I/R组,海马及皮质神经元凋亡增加,缺血再灌注损伤后皮质及海马神经元内GRP78阳性表达明显高于假手术组。bFGF组,海马及皮质神经元凋亡减少,皮质及海马神经元内GRP78表达较I/R组明显增加。结论 bFGF显著减少缺血神经元凋亡,上调脑缺血诱导的GRP78表达,对脑缺血再灌注海马及皮质神经元的具有保护作用。

Effects of bFGF on the expression of GRP78 in cerebral tissue during focal cerebral ischemia in rats

Objective To investigate the expression of GRP78 in hippocampus and cortex after cerebral ischemia and reperfusion in rats,explore the regulative effects and mechanism of basic fibroblast growth factor(bFGF)to GRP78 on brain tissue.Methods The model of middle cerebral arteries occlusion(MCAO) were performed with intraluminal filament blockade the expression of GRP78 and apoptosis cell in hippocampus and cortex was detected with immunohistochemical method,and TUNEL method.Results No apoptotic cells were observed in sham-operation group,the expression of GRP78 in the cortex and hippocampus tissue of rats was at low level in the sham-operation group.In ischemia-reperfusion group apoptotic neurons in ischemic region of cortex and hippocampus enhanced.The expression of GRP78 was increased in the model group.The decrease of apoptotic neurons in ischemic region of cortex and hippocampus were seen in bFGF treatment group.The expression of GRP78 in the cortex and hippocampus tissue of rats was markably increased in the treatment group than in the model group.Conclusion The results indicate that bFGF depress cell apoptosis,participate in the regulation of expression of GRP78 in ischemic neurons.