人参皂苷Rg3对食管癌放疗患者体外免疫增强作用的探讨

目的 探讨人参皂苷(Rg3)对食管癌放疗患者体外免疫有无增强作用,并初步探讨其相关机制。方法 选取2019年10月至2020年10月我院的30例食管癌放疗患者,分离、纯化其淋巴细胞,加入不同浓度的人参皂苷(Rg3),采用MTT法,来检测淋巴细胞的分裂增殖；通过流式细胞仪,检测T细胞亚群；以及ELISA法测定B细胞分泌的抗体。结果 人参皂苷(Rg3)处理食管癌放疗患者外周血后,诱导了淋巴细胞的增殖；CD4+比例增加、CD8+比例下降、CD4+/CD8+比值增大； IgM、IgG抗体分泌增多,与对照组比较均有统计学差异(P＜0.05),且呈剂量依赖性。结论 人参皂苷Rg3对食管癌放疗患者的体外免疫具有促进作用。

[1] Liu X, Zhang Z, Liu J, et al. Ginsenoside Rg3 improvescyclophosphamide-induced immunocompetence in Balb / c mice [J]. Int Immunopharmacol, 2019, 72(1): 98-111.

Objective To investigate whether Ginsenoside Rg3 can enhance the in vitro immunity of patients with esophageal cancer after radiotherapy, and to explore its related mechanism. Methods 30 patients with esophageal cancer treated by radiotherapy in our hospital from October 2019 to October 2020 were selected. Their lymphocytes were isolated and purified. The proliferation of lymphocytes was detected by MTT method, lymphocyte subsets were detected by flow cytometry, and antibodies were detected by ELISA. Results ginsenoside monomer Rg3 can significantly promote ConA and LPS induced lymphocyte proliferation. Ginsenoside monomer Rg3 can significantly increase the cd4+ ratio and cd4+/cd8+ ratio of lymphocyte subsets, significantly reduce the cd8+ ratio, and promote the secretion of IgM and IgG antibodies in peripheral blood lymphocytes after treating peripheral blood of esophageal cancer patients with radiotherapy. There are significant differences between ginsenoside monomer Rg3 and the control group (P < 0.05), and it is dose-dependent. Conclusion Ginsenoside Rg3 has in vitro immune enhancing effect on patients with esophageal cancer after radiotherapy.